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M13 a naturally occurring bacteriophage that contains a single-stranded circular genome of about 6400 Bases that infects only enteric bacteria containing the F-factor or producing the F pilus protein. The infecting virus is converted to a double-stranded circular replicative form that can be purified and digested with Restriction enzymes similar to bacterial plasmids. This virus has been manipulated in vitro for the construction of specialized cloning vectors that exist in a double-stranded form in the bacterial cell bit produce an infectious bacteriophage particle containing a single-stranded circular form, an excellent template for nucleotide sequence analysis.

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