Gel shift assay
(aka gel
mobility shift assay (GMSA), band shift assay (BSA), electrophoretic mobility
shift assay (EMSA)) A method by which one can determine whether a particular
protein preparation contains factors which bind to a particular DNA fragment.
When a radiolabeled DNA fragment is run on a gel, it shows a characteristic
mobility. If it is first incubated with a cellular extract of proteins (or with
purified protein), any protein-DNA complexes will migrate slower than the naked
DNA - a shifted band.
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