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5' or 3' end The nucleoside residues which form nucleic acids are joined by phosphodiester linkages between the 3' C atom of one ribose moiety and the 5' C atom of the next. Therefore each strand of DNA or RNA has a free 3' C at one end and a free 5' C at the other. The free 3' C normally carries a - OH group, and the 5' C a phosphate group.

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