jumping library
A tool for the mapping and sequencing of large stretches of a chromosome. Starting at one point in the polynucleotide sequence, a distal point is located without having to first sequence the intervening bases. The double-stranded DNA is divided into large fragments with a rare cutter and one end of each piece is ligated to a selective marker and cyclized, then cut with a frequent cutter. The marker allows isolation of the two ends of the original large piece without the intervening sequence.
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