phage display

A method for incorporation, into the DNA that encodes a phage coat protein, of a polypeptide, where it is available for a selection procedure; especially for production and selection of antibody (even anti-self antibody) fragments directed against a specific antigen. A library of VH or VL genes is produced by PCR using primers directed to constant regions that flank the variable region, cloned, and inserted into the gene for a phage coat protein that, when expressed, is available for cycles of growth and selection by adsorption on to an antigen-coated plate or beads.Makowski, L. (1994) Curr. Opin. Struct. Biol. 4, 225-230; Tramontano, A. and Sollazzo, M. (1995) Curr. Opin. Biotechnol. 6, 73-80; O'Neil, K.T. and Hoess, R.H. (1995) Curr. Opin. Struct. Biol. 5, 443-449

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