S1 nuclease mapping
A technique for identification of nucleotides at the 5'-end of an mRNA which, because it retains at that end its original triphosphate moiety, may be specifically labelled for sequencing by the Maxam-Gilbert technique. A restriction fragment that is complementary to the 5'-end of the mRNA protects it against S1 nuclease digestion, which is specific for single-stranded RNA and DNA; the only remaining polynucleotides therefore are the 5'-end of the mRNA and its unlabelled complement.
|
|
| GenScript Hot Products & Services: |
- Adenoviral Vector Services
The AV vectors provided by the GVC are standard E1- or E3-deleted AV vectors. Cloning plasmids are a... - Mas7
Amphiphilic peptide Mas7, a structural analogue of mastoparan is a known activator of heterotrimeric...
- Myelin Basic Protein (MBP) (68-82), guinea pig
Myelin basic protein (MBP)(68-82) induces experimental allergic encephalomyelitis. Myelin basic prot...
- OVA Peptide (257-264)
OVA peptide (257-264) is a class I (Kb)-restricted peptide epitope of OVA (amino acids 257-264), an...
| |
Return to Search Page
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send details to the Editorial Office at GenScript.
