V(D)J recombination
The process of assembling the DNA sequences that encode an immunoglobulin or a T-cell receptor (TCR), the cell-surface protein that recognizes an antigen. The process excises polynucleotide sequences between V (variable), D (diversity) and J (joining) sequences, with the assembly of a coding sequence that will encode a complete potential immunoglobulin or TCR. The mechanism involves excision at recombination signal sequences (RSSs) that are characterized at each end of the to-be-excised sequence by 23 and 12 bp sequences (the 12/23 rule) that will be fused together in the signal joint as the excised sequence is cyclized. The ends of the coding sequences, which were sealed as hairpin turns during the first phase of the splicing, are reopened, an overhang is filled in with the insertion of so-called P nucleotides, and ligated to form a coding joint.(see double-strand-break repair model)Bogue, M. and Roth, D.B. (1996) Curr. Opin. Immunol. 8, 175-180; Lewis, G.S.M. and Wu, G.E. (1997) Cell 88, 159-162; Oettinger, M.A. (1999) Curr. Opin Cell Biol. 11, 325-329; Landree, M.A., Wibbenmeyer, J.A. and Roth, D.B. (1999) Genes Dev. 13, 3059-3069
|
|
| GenScript Hot Products & Services: |
- Chemical Peptide Synthesis
GenScript peptide synthesis offer most competitive custom peptide synthesis.
... - Allatostatin I
Allatostatins (ASTs) of the Tyr/Phe-Xaa-Phe-Gly Leu/Ile-NH2 family are insect neuropeptides. Allatos...
- Bactenecin, bovine
Bactenecin is an antimicrobial peptide isolated from bovine neutrophils. Bactenecin is synthesized b...
- [Tyr4]-Bombesin
Bombesin is a 14 amino acid peptide originally isolated from the skin of a frog. Bombesin has two kn...
| |
Return to Search Page
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send details to the Editorial Office at GenScript.
