Cot unit

Nucleic Acids and Protein Calculations
Rare Codon Analysis Tools
Codon Usage Frequence Table
Codon Table
Restriction Enzyme Map Analysis
Primer Design Tool
Glossary of Biochemistry and Molecular Biology
Sequencing Primer Design
Real-Time PCR Primer Design
Oligo Calculation Tool
Chart of Amino Acid Structure

You are here: Resources » Online Tools » Glossary of Biochemistry and Molecular Biology

A compound unit used in measurement of the complexity of a DNA preparation. The rate of annealing of DNA is controlled by the second-order rate of nucleation, expressed as Co/C=1+k2Cot, where C is the concentration of single-stranded DNA at time t, Co is the original concentration of single-stranded DNA (both expressed as the molarity of nucleotides), and k2 is the second-order rate constant; often represented as a plot of C/Co against log(Cot). Cot½ taken from such plots is the value of Cot when C/Co=0.5.

GenScript Related Product&Service

DNA-PK Substrate

Taq DNA Polymerase

Lambda DNA/HindIII Marker - ready-to-use^TM

Pfu DNA Polymerase

Return to Search Page

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send details to the Editorial Office at GenScript.