competitive PCR
A variation of PCR to make it a semi-quantitative assay for a polynucleotide. A new restriction site is introduced into the polynucleotide to be assayed so that, when an unknown quantity of the original and a known amount of the modified template are used together, both will be copied but their products will be distinguishable. The products are quantified on the basis of binding a radiolabelled hybridization probe and incorporation of labelled primer or fluorescence of bound ethidium bromide; the ratio of their products is equated with the ratio of the original templates. Gilliland, G., Perria, S. and Bunn, H.F. (1990) in PCR Protocols: A Guide to Methods and Applications (Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., eds.), pp. 60-69, Academic Press, San Diego
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