2-D crystallography
A technique for low-resolution (10–20 Å) determination of shapes of proteins, especially large, complex proteins that are not amenable to crystallization by conventional techniques. A monomolecular layer of a lipid molecule is spread over an aqueous solution of the protein. A ligand that binds and orients the dissolved protein extends from monomolecular layer into the solution. The oriented two-dimensional protein molecular array is then analysed by electron diffraction crystallography.
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