You are here:  Resources » Bioinformatics Tools » Glossary of Biochemistry and Molecular Biology

delta restriction cloning A method for the use of a single primer to determine a DNA sequence longer than would normally be practical for a single sequencing attempt. After the first sequencing the bulk of the determined sequence is excised and the plasmid re-ligated, so that the primer is just upstream from the remaining undetermined sequence. The procedure may be repeated indefinitely until a large sequence is determined.

Return to Search Page

---

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send details to the Editorial Office at GenScript.