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dideoxynucleotide fingerprinting (ddF)

A method for the detection of mutation in a DNA fragment. The DNA serves as a template for replication using a mixture of four normal deoxynucleotides plus one dideoxyribonucleotide. electrophoresis on a non-denaturing gel shows shifted mobility for the mutant template products compared with those of the normal DNA template. (see also single-strand conformational polymorphism (SSCP))Sarkar, G., Yoon, H. and Sommer, S.S. (1991) genomics 13, 441-443
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