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displacement chromatography A column chromatographic method for separation and concentration of components of a solution. After adsorption of the sample to be separated on to the resin in a column, all bound molecules are displaced by a concentrated solution of a displacer, whose affinity for the stationary phase must be higher than that of any of the sample molecules. As the displacer sweeps the adsorbed molecules ahead of itself, each of these becomes a displacer for any molecule of lower affinity. Thus the original components are eluted in small volumes ahead of the displacer, in reverse order with regard to their affinities for the stationary phase.

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