dideoxynucleotides chain-terminating precursors of DNA synthesis that block further Polymerization when added to the end of the DNA strand by DNA Polymerase. These nucleotides lack a 3'-OH (hydroxyl) group necessary for continued 5'-to-3' DNA synthesis. dideoxynucleotides are used in molecular Biology for Sanger-type DNA sequencing, and in medicine as anti-retroviral drugs for the treatment of HIV infection (e.g., ddI, ddC, and AZT).
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