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gene cloning The production of a Lineage of cells all of which contain one kind of DNA fragment of interest derived from a population of many kinds of DNA fragments. Operationally by: inserting (recombining) a population of DNA molecules, known to contain the DNA of interest, into a population of vector DNA molecules in such a way that each vector molecule contains only a single DNA molecule from the original population; transforming a population of Host cells with the vector DNA recombinants such that each Host cell takes up only one vector; growing single Host cells separately (cloning) by plating at low density to form a collection of separate colonies; screening the colonies (clones) formed for the presence of the DNA of interest.

GenScript Related Product&Service Gene Synthesis DNA-PK Substrate Taq DNA Polymerase Pfu DNA Polymerase

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