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Stable transfection & cell line development services
Stable Transfection Services Quotation

Stable cell line development services for recombinant protein & antibody production

Stable cell lines are used in a variety of research applications like recombinant protein and antibody production, assay development, gene editing, and functional studies etc. At GenScript, we offer comprehensive stable cell line development capabilities that either begins with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging GenScript’s top quality stable cell line services.

 
Advanced Bioproduction Cell Line (SC1557)

Stable Bioproduction Cell Line (SC1503)
Steps
Gene synthesis & Subcloning (Optional)
Transient expression evaluation
Stable cell line development
Process Development
Deliverables
Target gene(s) in pUC57 & vector map
Expression report
Top 3 stable clones
(5 clones in case of SC1557)
Comprehensive report
Timeline
4 weeks
2-3 weeks
21-26 weeks
8-10 weeks
Description
  • GenScript synthesizes codon-optimized gene
  • Sub cloning into mammalian cell expression vector
  • Plasmid maxiprep for mammalian expression
  • Transfect cells with expression constructs using small scale expression system
  • Detect expression levels by ELISA and/or WB
  • Stable transfection and screening single cell clones
  • MTX amplification and screening
  • Subcloning of top 3 clones
  • Screening subclones with highest productivity
  • Stability test for 10 passages
  • Batch culture to evaluate final expression
  • Research cell bank preparation
  • Medium selection
  • Fed-batch process development
    • Physical compatibility experiment
    • Feed supplementation test

We offer GLP compliant services in both these categories

Flow chart of GenScript stable transfection & cell line development service

Flow chart of GenScript stable transfection & cell line development services

High quality cell lines start with high quality DNA

Our customers have the option of providing their own DNA for stable cell line generation purposes, however, GenScript recommends gene synthesis to ensure that we use high quality, codon optimized gene for the best expression levels.

Stable transfection

To create stable cell lines, DNA carrying the gene of interest has to be introduced into the cell with a stable transfection protocol. GenScript allows you to choose either plasmid-based stable transfection or lentivirus-based infection as your preferred stable transfection method.

Transient expression evaluation

To ensure that your protein of interest is expressed in the cells, we first characterize your protein expression by ELISA or Western Blot 48 hours after the transfection.

Stable cell line development

Transfected cells are grown in the appropriate selective media to generate stable pools. We can also subject the stable pools to additional Methotrexate (MTX) amplification and screening, in order to increase expression levels. The productivity and quality of the pools is evaluated up to 10 passages for the top 10 clones.

Clone picking

Top 3 clones from each pool are picked and evaluated further, based on their growth rates.

Cell line stability screening

Stability testing is a critical component of characterizing bioproduction cell lines. Stable cell lines in continuous culture suffer from genetic instability as the number of passages increases. Stability test will help you ensure the quality of your production cells over the intended passages.

Final deliverables, QC data and full report

We provide the sequence and construct report (in case of synthesized gene), top 3 stable clones and a comprehensive stable cell line generation report

Quotations and Ordering

Stable transfection and cell line development services Quotations & Ordering

For any other inquiries, please contact us using the details listed below. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.