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Stable transfection & cell line development services
Stable Transfection Services Quotation

Stable Cell Line Development Services For Recombinant Protein & Antibody Production

Stable cell lines are used in a variety of research applications such as recombinant protein and antibody production, assay development, gene editing, functional studies and more. At GenScript, we offer comprehensive stable cell line development capabilities that either begin with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging GenScript's top quality stable cell line services.

GenScript Production Stable Cell Line Service Options

GenScript Production Stable Cell Line Service Options
  • Host cells: CHO, CHO-K1, GS-knockout CHO-K1, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, U-2OS, or customer-specified cell line.
  • Genes: Antibodies, GPCRs, ion channels, proteases, kinases, transmembrane proteins and more.
  • Customized Service: Choose part or all of the following steps based on your specific needs.

Stable Cell Line Service Details

  • Service Description
  • Service Specifications
 
Steps
Gene synthesis & subcloning (optional)
Transient expression evaluation
Stable cell line development
Process development
(included with SC1557)
Deliverables
Target gene(s) in pUC57 & vector map
Expression report
Top 3 stable clones
(5 clones in case of SC1557)
Comprehensive report
Description
  • GenScript synthesizes codon-optimized gene
  • Subcloning into mammalian cell expression vector
  • Transfect cells with expression constructs using small scale expression system
  • Detect expression levels by ELISA and/or WB
  • Stable transfection and screening
  • MTX amplification and screening (SC1503 & SC1557)
  • Subcloning of top 3 clones
  • Screening subclones with highest productivity
  • Stability test for 10 passages
  • Batch culture to evaluate final expression
  • Research cell bank preparation
  • Medium selection
  • Fed-batch process development
    • –Physical compatibility
    • –Feed supplementation test

We offer GLP compliant services with all 3 packages.

Flow Chart Of GenScript Stable Transfection & Cell Line Development Service

Flow chart of GenScript stable transfection & cell line development services

High quality cell lines start with high quality DNA

Our customers have the option of providing their own DNA for stable cell line generation purposes, however, GenScript recommends gene synthesis to ensure that we use high quality, codon optimized genes for the best expression levels.

Stable transfection

GenScript allows you to choose from either GS or DHFR gene expression systems to construct your stable cell line. To start your project, DNA carrying the gene of interest must be introduced into the cell with a stable transfection protocol. Depending on your preference, we will use either plasmid-based stable transfection or lentivirus-based infection as your preferred stable transfection method.

Transient expression evaluation

To ensure that your protein of interest is expressed in the cells, we first characterize your protein expression by ELISA or Western Blot 48 hours after the transfection.

Stable cell line development

Transfected cells are grown in the appropriate selective media to generate stable pools. For packages using a DHFR expression system, we can also subject the stable pools to additional Methotrexate (MTX) amplification and screening, in order to increase expression levels (packages using a GS expression system do not require gene amplification). The productivity and quality of the pools is evaluated up to 10 passages for the top 10 clones.

Clone picking

Top 3 clones from each pool are picked and evaluated further, based on their growth rates.

Cell line stability screening

Stability testing is a critical component of characterizing bioproduction cell lines. Stable cell lines in continuous culture suffer from genetic instability as the number of passages increases. Stability test will help you ensure the quality of your production cells over the intended passages.

Final deliverables, QC data and full report

We provide the sequence and construct report (in case of synthesized gene), top 3 stable clones and a comprehensive stable cell line generation report

Stable Transfection Services Quotation

Quotations and Ordering


Stable transfection and cell line development services Quotations & Ordering

For any other inquiries, please contact us using the details listed below. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.