HAV P2C, Hepatitis A Virus Antigen, Recombinant
| Full Name |
HAV P2C, Hepatitis A Virus Antigen, Recombinant
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Abbreviated Name-1 |
Hepatitis A Virus P2C; Hepatitis A Virus; HAV P2C; |
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| Figures |
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| Reference |
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Zuckerman JN. Vaccination against hepatitis A and B: developments, deployment and delusions. Curr. Opin. Infect Dis. Oct 2006; 19(5):456-459.
Bizri AR, et al. Association between hepatitis A virus and Helicobacter pylori in a developing country: The saga continues. J. Gastroenterol Hepatol. Oct 2006; 21(10): 1615-1621.
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Description |
Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1,403-1,456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1,500-1,519 aa, includes almost the entire P3B, and the last domain, located at position 1,719-1,764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins. Hepatitis A Virus (HAV) P2C is E. coli derived recombinant protein. The protein contains the P2C immunodominant region. |
Source |
E. coli |
Purity |
Protein is >90% pure as determined by 10% PAGE (coomassie staining). |
Storage |
Protein is shipped at ambient temperature. Upon arrival, store at -20°C. |
Reconstitution |
1 mg/ml in 10 mM CBB, pH9.6, 0.1% SDS and 50% glycerol. |
Concentration |
1 mg/ml in 10 mM CBB, pH9.6, 0.1% SDS, 50% glycerol. |
Stability |
Five years frozen. One month in solution at room temperature. |
Specificity |
Immunoreactive with sera of HAV-infected individuals. |
Applications |
Antigen in ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems. |
Note |
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Related Products
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2 |
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Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems. |
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Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells. |
5 |
Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level. |
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