HBV HBe, Hepatitis B Virus Antigen, Recombinant

Full Name

Abbreviated Name-1

Hepatitis HBe; HBeAg; HBe;

Documents
Document-MSDS: 15621_20120306040124.PDF (PDF)
Figures
Reference
Peng XM, et al. High level of hepatitis B virus DNA after HBeAg-to-anti-HBe seroconversion is related to coexistence of mutations in its precore and basal core promoter. World J. Gastroenterol. May 2005; 11(20): 3131-3134. Yukimasa N, et al. A case of repeated seroconversion to HBe antibody due to co-infection with Mutant- and wild-type hepatitis B virus clones. Rinsho. Byori. Jan 2006; 54(1): 31-35.

Description

The Hepatitis 'e' antigen (HBeAg) is a peptide. It is usually detectable in the bloodstream when the hepatitis B virus is actively reproducing, which makes the patient much more infectious and at a greater risk of progression to liver disease. The exact function of this non-structural protein is unknown. However, it is thought that HBe may be influential in suppressing the immune systems response to HBV infection. HBeAg is generally detectable at the same time as HBsAg and disappears before HBsAg disappears. The presence of HBeAg in chronic infection is generally taken to indicate that HBV is actively reproducing and there is a higher probability of liver damage. In acute infections, HBeAg is generally only transiently present. Hepatitis B Virus (HBV) HBe is an E. coli derived recombinant. The protein contains the HBV HBe immunodominant region.

Source

E. coli

Purity

Protein is >95% pure as determined by 10% PAGE (Coomassie staining).

Storage

The protein is shipped at ambient temperature. Upon delivery, store the protein at -20°C.

Concentration

1 mg/ml, 25 mM Tris-HCl pH 8.0, 1.5 M urea, 50% glycerol

Stability

The protein will remain stable for five years if stored frozen and for one month if stored in solution at room temperature.

Specificity

The protein is immunoreactive with the sera of HBV-infected individuals.

Applications

The protein makes a good antigen in ELISA and western blots and an excellent antigen for the detection of HBV with minimal specificity problems

Note

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