HEV ORF2, Hepatitis E Virus Antigen, Recombinant

Full Name

Abbreviated Name-1

HEV-ORF2; Hepatitis E Virus Open Reading Frame proteins

Documents
Figures
Reference
Graff J, Torian U et al. A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus. J Virol. Jun 2006;80(12):5919-26. Surjit M, Jameel S, Lal SK. The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. J Virol. Jan 2004;78(1):320-8.

Description

Hepatitis E Virus (HEV) ORF2 is an E. coli-derived recombinant. The protein Hepatitis E Virus (HEV) ORF2 contains the HEV immunodominant regions from ORF2. Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.

Source

E. coli

Purity

Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Storage

Protein is shipped at ambient temperature. Upon arrival, store at -20°C.

Concentration

1 mg/ml in 25 mM Tris-HCl, 1 mM EDTA, 0.5 M urea, 50% glycerol.

Stability

Five years frozen. One month in solution at room temperature.

Specificity

Immunoreactive with sera of HEV-infected individuals.

Applications

Antigen in ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.

Note

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