| Full Name |
HCV NS3 Subtype 2b, Hepatitis C Virus Antigen, Recombinant
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Abbreviated Name-1 |
HCV-NS3-S2b; HCV-NS3-S-2b; Hepatitis C Virus-NS3-S2b; Hepatitis C Virus-NS3-S-2b |
| Documents |
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| Figures |
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| Reference |
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Lam AM, et al. Two novel conserved motifs in the hepatitis C virus NS3 protein critical for helicase action. J. Biol. Chem. Nov 2003; 278(45): 44514-44524.
Ogat S, et al. Correlation between secondary structure of an amino-terminal portion of the nonstructural protein 3 (NS3) of hepatitis C virus and development of hepatocellular carcinoma. Microbiol. Immunol. 2002; 46(8): 549-554.
Panigrahi AK, et al. Genotype determination of hepatitis C virus from northern India: identification of a new subtype. J. Med. Virol. Feb 1996; 48(2): 191-198.
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Description |
Hepatitis C Virus (HCV) NS3 Subtype 2b is an E. coli-derived recombinant from HCV subtype 2b. The protein contains the full-length HCV NS3 (c33c) immunodominant region, amino acids 1,192-1,459. |
Source |
E. coli |
Purity |
Protein is >95% pure as determined by 10% PAGE (coomassie staining). |
Storage |
Protein is shipped at ambient temperature. Upon arrival, store at -20°C. |
Concentration |
1 mg/ml in 25 mM Tris-HCl, 1 mM EDTA, 1.5 M urea, 50% glycerol. |
Stability |
Five years frozen. One month in solution at room temperature. |
Specificity |
Immunoreactive with sera of HCV-infected individuals, including sera with antibodies to NS3 secondary domains. |
Applications |
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems. |
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