HCV Nucleocapsid Genotype 1, Hepatitis C Virus Antigen, Recombinant

Full Name

Abbreviated Name-1

Hepatitis NG1; rHCVNG1

Documents
Figures
Reference
Boulant S, et al. Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b. J. Biol. Chem. Nov 2003; 278(46): 45785-45792. Castera L, et al. Effect of antiviral treatment on evolution of liver steatosis in patients with chronic hepatitis C: indirect evidence of a role of hepatitis C virus genotype 3 in steatosis. Gut. Mar 2004; 53(3): 420-424. Kalamvoki M, et al. The NS5A protein of the hepatitis C virus genotype 1a is cleaved by caspases to produce C-terminal-truncated forms of the protein that reside mainly in the cytosol. J. Biol. Chem. May 2006; 281(19): 13449-13462.

Description

E. coli derived recombinant. The protein Hepatitis C Virus (HCV) Nucleocapsid Genotype 1 contains the HCV core nucleocapsid immunodominant regions, amino acids 1-102. Hepatitis C Virus core proteins are purified by proprietary chromatographic techniques.

Source

E. coli

M.W.

39,918.16 Da (Including 26 Kd GST fusion)

Purity

Protein is >95% pure as determined by 10% PAGE (coomassie staining)

Storage

Protein is shipped at ambient temperature. Upon arrival, store at -20°C.

Concentration

1 mg/ml in 50 mM Tris. pH8.0, 5 mM EDTA

Stability

Five years frozen. 24 hrs in solution at room temperature.

Specificity

Immunoreactive with sera of HCV- infected individuals

Applications

Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Note

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