| Full Name |
Rubella Virus E2
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Abbreviated Name-1 |
Rubella Virus E2; |
| Documents |
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| Figures |
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| Reference |
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Law LM, Duncan R et al. Rubella virus E2 signal peptide is required for perinuclear localization of capsid protein and virus assembly. J Virol. Feb 2001;75(4):1978-83.
Yao J, Gillam S. A single-amino-acid substitution of a tyrosine residue in the rubella virus E1 cytoplasmic domain blocks virus release. J Virol. Apr 2000;74(7):3029-36.
Wilson KM, Di Camillo C et al. Humoral immune response to primary rubella virus infection. Clin Vaccine Immunol. Mar 2006;13(3):380-6.
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Description |
E. coli derived recombinant. The protein contains the Rubella virus E2 regions. The rubella virus (RV) structural proteins capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase. Among togaviruses, this feature is unique to RV. The E2 signal peptide has previously been shown to function as a membrane anchor for the capsid protein. |
Purity |
Protein is >95% pure as determined by 10% PAGE (coomassie staining). |
Storage |
Store at -20°C. Keep tightly closed. Store in a cool dry place. |
Concentration |
1 mg/ml in 20 mM imidazol, 8 M urea, 0.1 M NaCl |
Stability |
Five years frozen. One month in solution at room temperature. |
Specificity |
Immunoreactive with sera of Rubella virus-infected individuals |
Applications |
Antigen in ELISA and Western blots, excellent antigen for detection of Rubella virus with minimal specificity problems |
Note |
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