You are here: Products » Recombinant Proteins » Cytokines and Growth Factors » Granulocyte Colony Stimulating Factor, human

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Hot Products View by Category

Cat. No.	Name	Size	Price
Z00342-1	Granulocyte Colony Stimulating Factor (G-CSF), human	1 mg	$4680
Z00342-10	Granulocyte Colony Stimulating Factor (G-CSF), human	10 μg	$175
Z00342-50	Granulocyte Colony Stimulating Factor (G-CSF), human	50 μg	$600

Granulocyte Colony Stimulating Factor (G-CSF), human

Full Name

Granulocyte Colony Stimulating Factor (G-CSF), Human

Abbreviated Name-1

rHuG-CSF; G-CSF

Documents
Document-MSDS: 15088_20111227200143.PDF (PDF)
Document-COA: 15484_20120206004324.PDF (PDF)
Figures
Reference
Okamoto H, et al. A combination chemotherapy of carboplatin and irinotecan with granulocyte colony-stimulating factor (G-CSF) support in elderly patients with small cell lung cancer. Lung Cancer. Aug 2006; 53(2): 197-203. Zhang Y, et al. Synergistic effects of granulocyte-colony stimulating factor and macrophage-colony stimulating factor on recovery of donor hematopoietic cells in allogeneic bone marrow transplantation. Oncol. Rep. Aug 2006; 16(2): 367-371.

Description

Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.
Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da.

Source

E. coli

M.W.

18,800 Da

UV Scan

The maximal absorption wave is 278+/-3 nm.

Purity

Greater than 95% as determined by:
(a) Analysis by SEC-HPLC
(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel

Endotoxin Level

Less than 0.03ng/µg (0.3 IEU/µg) determined by LAL test.

Specific Activity

The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 6.0×10⁷ IU/mg.

Storage

Lyophilized Human Granulocyte Colony Stimulating Factor (G-CSF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution Human Granulocyte Colony Stimulating Factor (G-CSF) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.

Formulation

The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer.

Reconstitution

It is recommended to reconstitute the lyophilized rHuG-CSF in sterile 18 MΩ-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.

Quantitation

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.

Sequence analysis

The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser-Phe-Leu-Leu.

Note

;

Related Products

Cat. No.	Name	Size	Price
Z00349-10	Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human	10 μg	$100
Z00349-50	Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human	50 μg	$350
Z00349-1	Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), human	1 mg	$2925
Z00393-10	Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), human	10 μg	$170
Z00393-50	Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), human	50 μg	$640
Z00393-1	Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), human	1 mg	$3200


1	Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2	Bacterial Expression System: GenScript has developed BacPower^TM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3	Yeast Expression System: GenScript's proprietary YeastHIGH^TM technology combines the advantages of the bacterial and eukaryotic expression systems.
4	Baculovirus/Insect Cell Expression System: GenScript developed BacuVance^TM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5	Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.