IL-2, Human

Interleukin-2 (IL-2) is a Oglycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.

Price	$79.00
Cat. No.	Z00368-10
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Product Introduction Background Bottom Notes

Product Introduction

Species

Human

Protein Construction

IL-2 (Ala21-Thr153)
Accession # P60568

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 1 EU/μg of protein by LAL method

Biological Activity

The ED₅₀ as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.

Expression System

E. coli

Theoretical Molecular Weight

15.3 kDa

N-terminal Sequence Analysis

The sequence of the first five N-terminal amino acids wa determined and found to be Met-Ala-Pro-Thr-Ser-Ser.

Apparent Molecular Weight

15,300 Da

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H₂O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. Avoid repeated freeze-thaw cycles.

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Lane 1: 2 μg of IL-2, Human , non-reducing (NR)
Lane 2: 2 μg of IL-2, Human , reducing (R) )
> 95% as analyzed by SDS-PAGE »

Background

Target Background

Interleukin-2 (IL-2) is a Oglycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.

Synonyms

IL2; IL-2; TCGF; lymphokine; interleukin 2

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Shuhong Li , et al. Split-design approach enhances the therapeutic efficacy of ligand-based CAR-T cells against multiple B-cell malignancies. Nat Commun. (2024-11)
2. Yuxuan Chen , et al. An oncolytic virus–T cell chimera for cancer immunotherapy. Nat Biotechnol . (2024-02)
3. Xingxing Yuan, et al. Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors. Invest New Drugs. (2020)