| Full Name |
Macrophage Colony Stimulating Factor (M-CSF), Human
|
Abbreviated Name-1 |
Human recombinant M-CSF; Macrophage Colony Stimulating Factor |
| Documents |
|---|
| Figures |
|---|
| Reference |
|---|
Dauffy J, et al. The interferon-inducible gene, Ifi204, is transcriptionally activated in response to M-CSF, and its expression favors macrophage differentiation in myeloid progenitor cells. J Leukoc Biol. Jan 2006; 79(1): 173-183.
Oksjoki R, et al. OxLDL-IgG immune complexes induce survival of human monocytes. Arterioscler Thromb Vasc Biol. Mar 2006; 26(3): 576-583.
Clanchy FI, et al. Detection and properties of the human proliferative monocyte subpopulation. J Leukoc Biol. Apr 2006; 79(4): 757-766.
|
|
Description |
Macrophage Colony Stimulating Factor (M-CSF), human is produced by mesenchymal cells, such as fibroblasts, macrophages, and endothelial cells. The stimulating factor (M-CSF) stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. M-CSF binds to a specific high-affinity receptor. M-CSF is a disulfide-linked homodimer. |
Source |
Mesenchymal cells |
M.W. |
monomer 18,200 Da (reducing conditions), homodimer 30,300 Da (non-reducing conditions) |
Purity |
>97%, as determined by HPLC |
Endotoxin Level |
< 1.0 Endotoxin Unit (EU) per 1 µg as determined by the LAL method |
Specific Activity |
The ED50 < 5 ng/ml, determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells, has a specific activity of > 2×105 units/mg. |
Storage |
GenScript Lyophilized Human Macrophage Colony Stimulating Factor (M-CSF) will remain stable for two years if store at -20°C. After reconstitution, it may be stored at -70°C for up to one year. |
Formulation |
100 µg M-CSF, 10 mg HSA, 60 mg mannitol |
Reconstitution |
It is recommended that sterile PBS be added to prepare a stock solution of no less than 1 µg/ml. |
Sequence |
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS |
