HEV ORF2/ORF3, Hepatitis E Virus Antigen, Recombinant
| Full Name |
HEV ORF2/ORF3, Hepatitis E Virus Antigen, Recombinant
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Abbreviated Name-1 |
HEV ORF2/ORF3; |
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| Figures |
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| Reference |
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Graff J, et al. A bicistronic subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus. J. Virol. Jun 2006; 80(12): 5919-5926.
Tyagi S,et al. The phosphorylated form of the ORF3 protein of hepatitis E virus interacts with its non-glycosylated form of the major capsid protein, ORF2. J. Biol. Chem. Jun 2002; 277(25): 22759-22767.
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Description |
Hepatitis E Virus (HEV) ORF2/ORF3 is a E. coli derived recombinant protein, which contains the HEV immunodominant regions from ORF2 and ORF3. Hepatitis E virus (HEV) is a human RNA virus containing three open reading frames. Of these, ORF1 encodes the viral nonstructural polyprotein; ORF2 encodes the major capsid protein, which exists in a glycosylated and non-glycosylated form; and ORF3 codes for a phosphoprotein of undefined function, and ORF3 protein interacts with the ORF2 protein. The domains involved in this ORF2-ORF3 association have been identified and mapped, and a 25-amino acid region (residues 57-81) of the ORF3 protein is required for this interaction. ORF2-ORF3 interaction is dependent upon the phosphorylation at Ser(80) of ORF3, and the phosphorylated ORF3 protein preferentially interacts with the non-glycosylated ORF2 protein. |
Source |
E. coli |
Purity |
Protein is >95% pure as determined by 10% PAGE (coomassie staining). |
Storage |
Protein is shipped at ambient temperature. Upon arrival, store at -20°C. Five years frozen. One month in solution at room temperature. |
Concentration |
1 mg/ml in 25 mM Tris-HCl, 1 mM EDTA, 0.5 M urea, 50% glycerol. |
Specificity |
Immunoreactive with sera of HEV-infected individuals. |
Applications |
Antigen in ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems. |
Note |
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