Combined Hepatitis C Virus Antigen (HCV Antigen), Recombinant

Full Name

Abbreviated Name-1

Combined Hepatitis C Virus (HCV);

Documents
Figures
Reference
Bortolotti F, et al. Hepatitis C virus (HCV) genotypes in 373 Italian children with HCV infection: changing distribution and correlation with clinical features and outcome. Gut. Jun 2005; 54(6): 852-857. Boni S, et al. Hepatitis C virus core protein acts as a trans-modulating factor on internal translation initiation of the viral RNA. J. Biol. Chem. May 2005; 280(18): 17737-17748. Bair RM, et al. Prevalence and risk factors for hepatitis C virus infection among adolescents in detention. Archives of pediatrics & adolescent medicine. Nov 2005; 159(11): 1015-1018.

Description

E. coli derived recombinant. Combined Hepatitis C Virus (HCV) contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. The most diagnostically relevant proteins identified in this study were then contructed into one recombinant antigen.

Source

E. coli

Purity

Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Storage

Protein is shipped at ambient temperature. Upon arrival, store at -20°C.

Concentration

1 mg/ ml in 50 mM Na-PO₄, pH 8.5, 2.4 mM EDTA, 5 mM DTT,0.1% SDS.

Stability

Five years frozen. 24 hrs in solution at room temperature.

Specificity

Immunoreactive with sera of HCV- infected individuals.

Applications

Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Note

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