| Full Name |
HSA-IFN-α 2b, Human Serum Albumin And Interferon -α 2b
|
Abbreviated Name-1 |
HSA-IFN-α 2b; HSA-IFNa2b, HSA-IFN a2b |
|
Description |
At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19,000-26,000 Da and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end. GenScript Human Serum Albumin and Interferon (HSA-IFN)-α 2b produced in P. Pichia is a single non-glicosylated, polypeptide chain having a molecular mass of 85,700 Da. |
Source |
P. Pichia |
M.W. |
85,700 Da |
Purity |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC (b) Anion-exchange FPLC (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
Endotoxin Level |
Less than 0.1 ng/μg (IEU/μg) of HSA-IFN-α 2b |
Specific Activity |
HSA-IFN-α 2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5×107 IU/mg. |
Storage |
Lyophilized HSA-IFN-a2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSA-IFN-α 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to ass a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Formulation |
Lyophilized from a (1 mg/ml) solution in containing 5.55 mg sodium phosphate dibasic, 5.55 mg sodium phosphate monobasic buffer, 296 mg sucrose and 0.37 mg Tween 80. |
Reconstitution |
It is recommended to reconstitute the lyophilized HSA-IFN a2b in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Note |
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