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CD40 Ligand (CD40L/CD154/TRAP), Human



Cat. No.
Name
Size   
Price
Selected
Z02022-10
10 μg
$65
Z02022-50
50 μg
$159
Z02022-1
1 mg(2x500 μg)
$1,049

Protein CD40 Ligand (CD40L/CD154/TRAP), Human
Synonyms
CD40 Ligand, CD154, TRAP, TNFSF5, gp39
Documents
CD40L/CD154/TRAP, HumanDocument-MSDS: 15114_20111229220457.PDF (PDF)
Species
Human
Purity
Greater than 95.0% as determined by:
1. Analysis by RP-HPLC.
2. Anion-exchange FPLC.
3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Endotoxin Level
Less than 0.1 ng/µg (1 EU/µg) of recombinant human CD40 Ligand (CD40L) as determined by LAL test.
Biological Activity
Recombinant human CD40 Ligand (CD40L/CD154/TRAP) is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.
Source
E. coli
Molecular Weight
16,308 Da
Description
CD40 Ligand (CD40L/CD154/TRAP) is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-γ, TNF-α, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes.
Recombinant Human CD40 Ligand (CD40L/CD154/TRAP) produced in E. coli is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16,308 Da.
Formulation
The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer.
Reconstitution
It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage
Lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD40L should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Sequence analysis
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Lys-Gly-Asp.
Document-COA
  • 158931_2_Z02022_COA.pdf
  • 168063_2_Z02022_COA.pdf
  • 175146_2_Z02022_COA.pdf
  • 182904_11_Z02022_COA.pdf
  • 182904_12_Z02022_COA.pdf
  • C50111211_Z02022_COA.pdf
  • C50621310_Z02022_COA.pdf
  • C51241210_Z02022_COA.pdf
  • C55271212_Z02022_COA.pdf
  • C55991308_Z02022_COA.pdf
  • C58421310_Z02022_COA.pdf
  • C59961210_Z02022_COA.pdf
  • 175146_2_Z02022_COA.pdf
  • 182904_11_Z02022_COA.pdf
  • 182904_12_Z02022_COA.pdf
  • C50111211_Z02022_COA.pdf
  • C50621310_Z02022_COA.pdf
  • C51241210_Z02022_COA.pdf
  • C55271212_Z02022_COA.pdf
  • C55991308_Z02022_COA.pdf
  • C58421310_Z02022_COA.pdf
  • C59961210_Z02022_COA.pdf
  • Citation
    Oertelt S, et al. Soluble CD40L in plasma of patients with primary biliary cirrhosis. Ann. N. Y. Acad. Sci. Jun 2005; 1051: 205-210.

    Danese S, et al. TNF-alpha blockade down-regulates the CD40/CD40L pathway in the mucosal microcirculation: a novel anti-inflammatory mechanism of infliximab in Crohn's disease. J. Immunol. Feb 2006; 176(4): 2617-2624.

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