CMV Pp28 (UL99), CytomegaloVirus Antigen, Recombinant

Full Name

Abbreviated Name-1

CMV Pp28 (UL99);

Documents
Figures
Reference
Lazzarotto T, et al. The tegument protein ppUL25 of human cytomegalovirus (CMV) is a major target antigen for the anti-CMV antibody response. J. Gen. Virol. Feb 2001; 82(Pt 2): 335-338. Wang Z, et al. Attenuated poxviruses generate clinically relevant frequencies of CMV-specific T cells. Blood. Aug 2004; 104(3): 847-856. Seo JY, Britt WJ. Sequence requirements for localization of human cytomegalovirus tegument protein pp28 to the virus assembly compartment and for assembly of infectious virus. J. Virol. Jun 2006; 80(11): 5611-5626.

Description

E. coli derived recombinant. CytomegaloVirus (CMV) Pp28 (UL99) contains the CMV Pp28 (UL99) immunodominant regions. The Pp28 protein is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope. In the infected cell, Pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane. CytomegaloVirus (CMV) Pp28 (UL99) is purified by proprietary chromatographic techniques.

Source

E. coli

Purity

Protein is >95% pure as determined by 10% PAGE (coomassie staining).

Storage

Protein is shipped at ambient temperature. Upon arrival, store at -20°C.

Concentration

1 mg/ml in 25 mM Tris-Hcl pH7.2, 1 mM EDTA, 50% glycerol.

Stability

Five years frozen. One month in solution at room temperature.

Specificity

Immunoreactive with sera of CMV-infected individuals.

Applications

Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.

Note

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