| Full Name |
Ribonuclease Inhibitor
|
Abbreviated Name-1 |
Ribonuclease Inhibitor; |
|
Description |
Ribonuclease Inhibitor is a recombinant human placental protein which specifically inhibits ribonuclease (RNase) A, B and C. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA polymerase, AMV or M-MLV reverse transcriptase, or phage RNA polymerases (SP6, T7, or T3). |
Source |
E. coli |
Purity |
>95% |
Specific Activity |
One unit is defined as the amount of RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A. |
Storage |
Store at -20°C. Avoid exposure to frequent temperature changes. |
Concentration |
40,000 units/ml in 20 mM HEPES-KOH (pH 7.5), 50 mM KCl, 8 mM DTT and 50% glycerol. |
Applications |
Applied in procedures where RNase contamination constitutes a problem:
* RT-PCR;
* cDNA synthesis;
* In vitro transcription/translation;
* RNA synthesis;
* Increase yield and activity of polysomes;
* Improvement of in vitro virus replication;
* Improvement of RNA translation in homologous systems;
* Preparation of RNase-free antibody. |
Note |
; |
Document-MSDS: 7367_20081028050541.PDF (PDF)