| Full Name |
T4 Gene 32 Protein
|
Abbreviated Name-1 |
T4 gp32; |
|
Description |
T4 Gene 32 Protein is a single-stranded DNA binding protein from bacteriophage T4 which cooperatively binds to transiently formed ssDNA regions. It can eliminate pausing when sequencing through strong secondary structures and improve the specificity and yield of PCR products. T4 Gene 32 Protein has been widely used in studies of DNA-protein interactions and in the building of single-stranded DNA marking regions. |
Source |
E. coli |
Purity |
Greater than 95% pure as determined by SDS-PAGE. |
Storage |
Store at -20°C or below. |
Concentration |
10 µg/µl (determined by Bradford protein assay) in 20 mM Tris-HCl, 100 mM NaCl, 0.5 mM Dithiothreitol, 1 mM EDTA, 50% Glycerol, pH 8.0 at 25°C. |
Applications |
Increase yield and specificity of PCR.
Create and stabilize ssDNA marking regions.
Improve sequencing through regions with complex secondary structures. |
Note |
; |
Document-MSDS: 7377_20081028053626.PDF (PDF)