| Full Name |
Purified Protein A
|
Abbreviated Name-1 |
Purified Protein A; |
|
Description |
Purified protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. With a molecular weight of 35,000 Da, it is made up of a single polypeptide chain containing five Ig-binding regions. Purified protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Purified protein A binds to most human and mouse IgG subclasses (e.g. human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken IgY. To reconstitute the powder, for 10 mg size (Z02201-10), add 0.8ml water and 0.2ml 1xPBS, and the final concentration is 10mg/ml in 1x PBS. Please scale up accordingly for other sizes, such as Z02201-50 or Z02201-500. |
Source |
Expressed and purified from E. coli |
M.W. |
35,000 Da |
Purity |
This product is highly purified, SDS-PAGE and HPLC analyses revealed its purity of more than 95%. |
Storage |
Store the lyophilized powder at 4°C for several weeks, for long-term storage, store it at -20°C. |
Formulation |
Lyophilized from 1X PBS |
Applications |
Purified protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, β-galactosidase, alkaline phosphatase), biotin, and colloidal gold. These conjugates can be used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications. In addition, purified protein A may be immobilized on a solid support such as agarose or acrylic beads to purify polyclonal or monoclonal immunoglobulins. It can also be used in immunoprecipitation assays. |
 |
1 |
Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 . |
2 |
Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins. |
3 |
Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems. |
4 |
Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells. |
5 |
Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level. |
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