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Cat. No.
Name
Size   
Price
Selected
Z02201-10
10 mg
$79.00
Z02201-50
50 mg
$350.00
Z02201-500
500 mg
$2500.00

Full Name Purified Protein A
Abbreviated Name-1
Purified Protein A;
Documents
Purified Protein ADocument-EXAMPLE: 9518_20090316233042.JPG (JPG)
Purified Protein ADocument-MSDS: 9619_20090422212304.PDF (PDF)
Purified Protein ADocument-COA: 9636_20090423034826.PDF (PDF)
Purified Protein ADocument-COA: 14218_20111114035406.PDF (PDF)
Purified Protein ADocument-COA: 14219_20111114035406.PDF (PDF)
Figures
Reference
Description
Purified protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. With a molecular weight of 35,000 Da, it is made up of a single polypeptide chain containing five Ig-binding regions. Purified protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Purified protein A binds to most human and mouse IgG subclasses (e.g. human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken IgY. To reconstitute the powder, for 10 mg size (Z02201-10), add 0.8ml water and 0.2ml 1xPBS, and the final concentration is 10mg/ml in 1x PBS. Please scale up accordingly for other sizes, such as Z02201-50 or Z02201-500.
Source
Expressed and purified from E. coli
M.W.
35,000 Da
Purity
This product is highly purified, SDS-PAGE and HPLC analyses revealed its purity of more than 95%.
Storage
Store the lyophilized powder at 4°C for several weeks, for long-term storage, store it at -20°C.
Formulation
Lyophilized from 1X PBS
Applications
Purified protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, β-galactosidase, alkaline phosphatase), biotin, and colloidal gold. These conjugates can be used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications. In addition, purified protein A may be immobilized on a solid support such as agarose or acrylic beads to purify polyclonal or monoclonal immunoglobulins. It can also be used in immunoprecipitation assays.
1
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