| Full Name |
AMPK(α2/β1/γ1), Active (Mammalian Expression System)
|
Abbreviated Name-1 |
AMPK211, AMPK(A2/B1/G1); |
|
Description |
Recombinant human trimeric protein complex composed of full length PRKAA2, PRKAB1 and Flag-tagged PRKAG1, co-expressed in HEK293. The complex was activated in vitro with histidine-tagged CAMKK1. |
Source |
Recombinant enzyme co-expressed in HEK293 |
Purity |
>80% as estimated by a Coomassie blue-stained SDS-PAGE gel. |
Specific Activity |
601 nmole of phosphate transferred to SAMS peptide (HMRSAMSGLHLVKRR) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.029 μg/ml. Activated 3.2 times by 100 μM AMP. |
Storage |
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. If properly stored at -80°C, this product is guaranteed for 6 months from date of purchase. |
Concentration |
0.680 mg/ml, as determined by Bradford protein assay with BSA as a standard. |
Applications |
Kinase Assay, Western Blot |
Note |
; |
Document-MSDS: 10839_20090923034751.PDF (PDF)