Mitogen-Activated Protein Kinase Kinase 1 (MEK1, inactive)
*This product has been discontinued! *
|Description||C-terminal 6His-tagged, recombinant full-length human MEK1 with a K97R mutation expressed in E. coli. Purity >80% by SDS PAGE and Coomassie blue staining. Calculated MW = 44.3 kDa.|
|Biological Activity||MEK1 (K97R), inactive is a suitable substrate for Raf-1, but is not activated upon phosphorylation.|
|Molecular Weight||Calculated MW = 44.3 kDa.|
|Concentration||0.386 mg/ml, as determined by Bradford protein assay with BSA as a standard.|
|Purity||Calculated MW = 44.3 kDa.|
50 μg of recombinant protein in 130 μl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA,0.03%Brij-35, 10% glycerol, 0.1% 2-mercaptoethanol, 0.2mM PMSF. Frozen solution.
Storage: Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
|Note||; Order a unit now and get it within 4-6 weeks.|
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