0.386 mg/ml, as determined by Bradford protein assay with BSA as a standard.
Calculated MW = 44.3 kDa.
50 μg of recombinant protein in 130 μl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA,0.03%Brij-35, 10% glycerol, 0.1% 2-mercaptoethanol, 0.2mM PMSF. Frozen solution.
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.