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Mitogen-Activated Protein Kinase Kinase 1 (MEK1, inactive)



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*This product has been discontinued! *

Protein Mitogen-Activated Protein Kinase Kinase 1 (MEK1, inactive)
Synonyms
MEK1;
Documents
Purity
Calculated MW = 44.3 kDa.
Biological Activity
MEK1 (K97R), inactive is a suitable substrate for Raf-1, but is not activated upon phosphorylation.
Source
E. coli.
Molecular Weight
Calculated MW = 44.3 kDa.
Description
C-terminal 6His-tagged, recombinant full-length human MEK1 with a K97R mutation expressed in E. coli. Purity >80% by SDS PAGE and Coomassie blue staining. Calculated MW = 44.3 kDa.
Storage
Storage Buffer: 50 μg of recombinant protein in 130 μl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA,0.03%Brij-35, 10% glycerol, 0.1% 2-mercaptoethanol, 0.2mM PMSF. Frozen solution.
Storage: Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
Concentration
0.386 mg/ml, as determined by Bradford protein assay with BSA as a standard.
Note
; Order a unit now and get it within 4-6 weeks.
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