| Full Name |
Macrophage Colony Stimulating Factor (M-CSF), Human
|
Abbreviated Name-1 |
Macrophage Colony Stimulating Factor (M-CSF), Human; |
|
Description |
Macrophage-Colony Stimulating Factor (M-CSF), also known as CSF-1, is a four ª helical bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation.
Macrophage-Colony Stimulating Factor (M-CSF) is also essential for the survival and proliferation of osteoclast progenitors. Macrophage-Colony Stimulating Factor (M-CSF) also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. |
Source |
Baculovirus infected Silkworm |
M.W. |
Approximately 42.0 kDa, a covalently linked homodimer, of two 21.0 kDa polypeptide monomers (1-149 amino acid of human M-CSF). |
Purity |
>95% by SDS-PAGE and HPLC analyses. |
Endotoxin Level |
Less than 1 EU/ug of Recombinant Human Macrophage-Colony Stimulating Factor (M-CSF) as determined by LAL method. |
Specific Activity |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine M-NSF-60 cells is less than 3.0 ng/ml. |
Storage |
Lyophilized Recombinant Human Macrophage-Colony Stimulating Factor (M-CSF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution Recombinant Human Macrophage-Colony Stimulating Factor (M-CSF) should be stored at 2-8°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2µm filtered solution in 20mM PB, containing 1% HSA and 3% Mannitol. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Sequence |
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDKACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ |
Note |
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Document-COA: 14569_20120206020219.PDF (PDF)