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Growth Hormone (GH), Human



Cat. No.
Name
Size   
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Z02791-100
100 μg
$135

Protein Growth Hormone (GH), Human
Synonyms
Growth Hormone (GH), Human;
Documents
Growth Hormone (GH), HumanDocument-MSDS: 15308_20120104035409.PDF (PDF)
Purity
>96% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuGH as determined by LAL method.
Biological Activity
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0×107 IU/mg.
Source
Escherichia coli.
Amino Acid Sequence
FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ
KYSFLQNPQTSLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV
QFLRSVFANSLVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK
QTYSKFDTNSHNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG
F
Molecular Weight
Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids
Description
Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones-GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin and leptin.4 The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution with 5mM NaHCO3, pH 7.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
This lyophilized preparation is stable for several weeks at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Document-COA
  • Z02791_R50881204.pdf
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