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MIP-1 beta /CCL4, Human



Cat. No.
Name
Size   
Price
Selected
Z02831-1
1 mg
$2,400
Z02831-10
10 μg
$157

Full Name MIP-1 Beta /CCL4, Human
Abbreviated Name-1
MIP-1 beta /CCL4, Human;
Documents
MIP-1 beta /CCL4, HumanDocument-MSDS: 15362_20120105034106.PDF (PDF)
MIP-1 beta /CCL4, HumanDocument-COA: R51131204_Z02831_COA.pdf (pdf)
Figures
Reference
Description
Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and mouse MIP-1 alpha and MIP-1 beta are active on human and mouse hematopoietic cells.
Source
Escherichia coli.
M.W.
7.6 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Purity
>96% by SDS-PAGE and HPLC analyses.
Endotoxin Level
Less than 0.2EU/ug of rHuMIP-1 beta /CCL4 as determined by LAL method.
Specific Activity
Determined by its ability to chemoattract human blood monocytes using a concentration range of 5.0-20.0 ng/ml,corresponding to a specific activity of > 5.0×104 units/mg.
Storage
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in 20mM Tris, 500mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Sequence

APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV
VFQTKRGKQV CADPSESWVQ EYVYDLELN
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