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Bone Morphogenetic Protein-2 (BMP-2), Human



Cat. No.
Name
Size   
Price
Selected
Z02913-50
50 µg
$574
Z02913-1
1 mg
$4,160
Z02913-10
10 μg
$157
Z02913-100
100 µg
$1,040

Full Name Bone Morphogenetic Protein-2 (BMP-2), Human
Abbreviated Name-1
BMP-2, BMP2A, Bone morphogenetic protein 2, BMP-2A, BMP2
Documents
Bone Morphogenetic Protein-2 (BMP-2), HumanDocument-COA: COA of Human BMP-2.pdf (pdf)
Bone Morphogenetic Protein-2 (BMP-2), HumanDocument-MSDS: MSDS of Human BMP-2.pdf (pdf)
Bone Morphogenetic Protein-2 (BMP-2), HumanDocument-COA: COA of Human BMP-2.pdf (PDF)
Bone Morphogenetic Protein-2 (BMP-2), HumanDocument-COA: COA of Human BMP-2_P20011210_.pdf (PDF)
Bone Morphogenetic Protein-2 (BMP-2), HumanDocument-COA: P50011308_Z02913_COA.pdf (PDF)
Figures
Reference
Sampath, T.K. et al., Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily. J Biol Chem. 1990 Aug 5;265(22):13198-205.

Heng S. et al., Posttranslational activation of bone morphogenetic protein 2 is mediated by proprotein convertase 6 during decidualization for pregnancy establishment. Endocrinology. 2010 Aug;151(8):3909-17. Epub 2010 Jun 16.

Celeste, A. J. et al., Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9843-7.

Description
Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.

Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Source
E. coli
M.W.
26 kDa, observed by non-reducing SDS-PAGE
Purity
> 95% as analyzed by non-reducing SDS-PAGE.
Endotoxin Level
< 1 EU/μg, determined by LAL method.
Specific Activity
ED50 < 1 μg/ml, measured by alkaline phosphatase induction assay using C2C12 cells.
Storage
Lyophilized recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhBMP-2 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Formulation
Lyophilized after extensive dialysis against 50 mM acetic acid.
Reconstitution
Reconstituted in 20 mM AcOH or 5 mM HCl. The solubility should be at 100 μg/ml.
Sequence

Gln283-Arg396 (accession number:P12643). Expressed
with an N-terminal Met.
Note
1
Custom Recombinant Protein Services: Guaranteed E. coli Expression Package-3 mg purified soluble protein from $2,200 .
2
Bacterial Expression System: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins.
3
Yeast Expression System: GenScript's proprietary YeastHIGHTM technology combines the advantages of the bacterial and eukaryotic expression systems.
4
Baculovirus/Insect Cell Expression System: GenScript developed BacuVanceTM platform for the secretion of recombinant proteins from baculovirus-infected insect cells.
5
Mammalian Cell Expression System: The proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.