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Bone Morphogenetic Protein-2 (BMP-2), Human


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Cat. No.
Product Name
Size
Price
Quantity
Z02913-50 BMP-2, Human
50 µg
$268.00
Z02913-100 BMP-2, Human
100 µg
$479.00
Z02913-10 BMP-2, Human
10 μg
$85.00
Z02913-1 BMP-2, Human
1 mg
$1600.00



Synonyms BMP-2, BMP2A, Bone morphogenetic protein 2, BMP-2A, BMP2
Description Human Bone Morphogenetic Protein-2 (BMP-2) is a bone-growth regulatory factor and belongs to the transforming growth factor-beta (TGF-beta) superfamily. Human Bone Morphogenetic Protein-2 (BMP-2) is synthesized as large precursor molecule (Met1-Arg396, with a signal peptide from Met1 to Gly23), propeptide (Leu24-Arg282) of which is cleaved by PCSK5 (Proprotein Convertase Subtilisin/Kexin type 5). The active form consists of a dimer of two identical proteins which are linked by a disulfide bond at Cys360. It plays an important role in the development of bone and cartilage, cardiac cell differentiation and epithelial to mesenchymal transition. It is also involved in the hedgehog pathway, TGF-beta signaling pathway, and in cytokine-cytokine receptor interaction.

Recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 115 amino acids. A fully biologically active molecule, rhBMP-2 has a molecular mass of 26 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Amino Acid Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH
AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC
VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Source E. coli
Species Human
Biological Activity Assay #1: Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells, The ED50 for this effect is typically 0.07-0.2 µg/mL.
Assay #2: Measured by its ability to induce alkaline phosphatase production by C2C12 cells, The ED50 for this effect is typically 0.2-1 µg/mL.

Molecular Weight 26 kDa, observed by non-reducing SDS-PAGE
Formulation Lyophilized after extensive dialysis against 50 mM acetic acid.
Reconstitution Reconstituted in 20 mM AcOH or 5 mM HCl. The solubility should be at 100 μg/ml.
Purity > 95% as analyzed by non-reducing SDS-PAGE and HPLC analyses
Endotoxin Level < 1 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhBMP-2 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note

BMP-2, Human

BMP-2, Human
BMP-2, Human

Biological Activity
BMP-2, Human (Cat. No. Z02913) induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.07-0.20 ug/mL.

BMP-2, Human
BMP-2, Human

Biological Activity
BMP-2, Human (Cat. No. Z02913) induce alkaline phosphatase production by C2C12 cells. The ED50 for this effect is typically 0.10-0.80 ug/mL.

BMP-2, Human
BMP-2, Human

2 μg of BMP-2, Human (Cat. No. Z02913) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

BMP-2, Human

BMP-2, Human

BMP-2, Human


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You can visit "Citations Database" for more citations. A Clark., et al. Mechanical Properties and Dual Drug Delivery Application of PLGA Scaffolds Fabricated with a Poly (β-amino ester) Porogen. Acta Biomater. 2014Jan.

You can visit "Citations Database" for more citations. E Alegre-Aguarón., et al. Growth Factor Priming Differentially Modulates Components of the Extracellular Matrix Proteome in Chondrocytes and Synovium-Derived Stem Cells. PLoS One. 2014Feb.;9(2):e88053

You can visit "Citations Database" for more citations. Cushnie EK., et al. Simple Signaling Molecules for Inductive Bone Regenerative Engineering. PLoS One. 2014July;9(7):e101627

You can visit "Citations Database" for more citations.







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