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VEGF164, Rat (P. pastoris-expressed)

*This product has been discontinued!*
Recombinant rat Vascular Endothelial Growth Factor A164 (rrVEGF-A164) produced in P. pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rrVEGF-A164 has a molecular mass of 38 kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z02917
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Product Introduction
Species Rat
Protein Construction

Expressed with an N-terminal Met.

VEGF164 (Ala27-Arg190)
Accession # P16612-2
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity ED50 < 4.0 ng/ml, measured by cell proliferation assay of HUVEC.
Expression System P. pastoris
Apparent Molecular Weight ~38 kDa, on SDS-PAGE under non-reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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Background
Target Background Vascular Endothelial Growth Factor A164 (VEGF-A164), a member of the cysteine knot growth factor, is one of major isoforms of VEGF-As. VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties. During maturation, rat VEGF-A is alternatively spliced to generate rVEGF-A120, rVEGF-A164 and rVEGF-A188 which correspond to hVEGF-A121, hVEGF-A165 and hVEGF-A189 in human, respectively (the numbers designate the amino acid residues). The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR or to the non-tyrosine kinase neuropilin receptors NRP1/2.
Synonyms Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen; VEGF-164; Vascular Permeability Factor; VPF
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.