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TNF-α, Mouse (P. pastoris-expressed)

Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.
$100.00
Z02918-20

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Product Introduction
Species Mouse
Protein Construction
TNF-α (Ser84-Leu235)
Accession # P06804
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 1 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.01 ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of > 1.0 × 108 U/mg.
Expression System P. pastoris
Apparent Molecular Weight ~17 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
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TNF-α, Mouse (P. Pastoris-expressed)

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TNF-α, Mouse (P. Pastoris-expressed)

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TNF-α, Mouse (P. Pastoris-expressed)

ED50 < 0.01 ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of > 1.0 × 108 units/mg. »

TNF-α, Mouse (P. Pastoris-expressed)

Lane 1: 2 μg of TNF-α, Mouse (P. pastoris-expressed), reducing (R)
> 95% as analyzed by SDS-PAGE »

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Background
Target Background Tumor necrosis factor alpha (TNF-α) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-α occurs as a membrane-anchored form. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species.
Synonyms TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Citations
Lina Abou Saleh, et al. Ablation of PDE4B protects from Pseudomonas aeruginosa-induced acute lung injury in mice by ameliorating the cytostorm and associated hypothermia. FASEB J. (2021-09)
Zhen Wang , et al. Endothelin promotes colorectal tumorigenesis by activating YAP/TAZ. Cancer Research,. (2018)
Tang Alan T, et al. Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature. (2017)
Jiang S, et al. Gastric Bypass Surgery Reverses Diabetic Phenotypes in Bdnf-Deficient Mice. Am J Pathol. (2016)