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Fibroblast Growth Factor-8c (FGF-8c), Mouse



Cat. No.
Name
Size   
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Z03075-1
1 mg
$1,500

Protein Fibroblast Growth Factor-8c (FGF-8c), Mouse
Synonyms
Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, Fgf8
Documents
FGF-8c, MouseDocument-MSDS: Z03075_MSDS.pdf (pdf)
Species
Mouse
Purity
> 95% by SDS-PAGE and HPLC analyses.
Endotoxin Level
< 0.2 EU/μg, determined by LAL method.
Biological Activity
ED50 < 150 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 6.7× 103 units/mg.
Source
E. coli
Amino Acid Sequence
MQVRSAAQKR GPGAGNPADT LGQGHEDRPF GQRSRAGKNF
TNPAPNYPEE GSKEQRDSVL PKVTQRHVRE QSLVTDQLSR
RLIRTYQLYS RTSGKHVQVL ANKRINAMAE DGDPFAKLIV
ETDTFGSRVR VRGAETGLYI CMNKKGKLIA KSNGKGKDCV
FTEIVLENNY TALQNAKYEG WYMAFTRKGR PRKGSKTRQH
QREVHFMKRL PRGHHTTEQS LRFEFLNYPP FT
Molecular Weight
28.2 kDa, observed by reducing SDS-PAGE.
Description
Fibroblast Growth Factor 8c (FGF-8c) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. In different species, e.g. human and mouse, FGF-8 has 8 different isoforms, from FGF-8a to FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, thus conduct different signaling cascade pathways. FGF-8 has very widespread expression pattern during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression; on the other hand, FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.
Recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) produced in E. coli is a single non-glycosylated polypeptide chain containing 247 amino acids. A fully biologically active molecule, rmFGF-8c has a molecular mass of 28.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Formulation
Lyophilized after extensive dialysis against PBS.
Reconstitution
Reconstituted in ddH2O at 100 μg/mL.
Storage
Lyophilized recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-8c should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note
For research use only
Document-COA
  • P50011306_Z03075_COA.pdf
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