Protein A, G and A/G MagBeads

GenScript Protein A, G and A/G MagBeads are developed for quick, convenient and reproducible immunoprecipitation(IP) and small scale purification of immunoglobulin. By covalently pre-coupling recombinant Protein A, G and A/G to the surface of superparamagnetic beads, these MagBeads provide users simple, easy and time-saving procedures for immunomagnetic separation.

Due to the elimination of non-IgG binding domains and other unknown function fragments, the recombinant Protein A, G and A/G both display high affinity for subclasses of IgG from many species including human, rabbit and mouse etc. This permits the purification of IgGs from cell culture supernatants, serum and ascites or other starting samples. In addition, Protein A, G and A/G MagBeads can also be used to immunoprecipitate target proteins from crude samples using selected primary antibody. To prevent co-elution of the antibody, it is necessary to chemically cross-link the antibody to protein A, G and A/G coated beads prior to the immunoprecipitation steps.

By applying magnetic separation technology, the Protein A, G and A/G MagBeads supply researchers fast, easy and efficient IP/isolation procedures. The additional device you need is just a magnetic separation rack. All the steps take place in a single tube. No columns, centrifugation and pre-treatment are needed. Extremely gentle magnetic-handling ensures no loss of your target IgGs or protein and keeps them intact during whole procedures. All the advantages make Protein A, G and A/G MagBeads the perfect tools for immunoprecipitation and small-scale IgG purification.

Product Details
Examples

Table 1. Properties of the recombinant Protein A, G and L on Resins

	Protein A	Protein G	Protein A/G
Source	Staphylococcus aureus	Streptococcus
Molecular weight	43 kDa	22 kDa	43kDa
Numbers of IgG(Ig) binding sites	5	3	4 of Protein A and 2 of Protein G
IgG Binding capacity	2.5 mg porcine IgG per ml settled beads	10 mg goat IgG per ml settled beads	10 mg goat or porcine IgG per ml settled beads

Fig.2. Purification of Rabbit IgG from Rabbit serum and Immunoprecipitation of His-Tagged protein from bacterial lysate using GenScript Protein A MagBeads. Lane M, Protein Molecular Weight Standard. Lane 1, Flowthrough of rabbit serum. Lane 2, Purified rabbit IgG from serum. Lane 3, Bacterial lysate expressing His-tagged protein. Lane 4, Immunoprecipitated His-tagged protein, 32kDa (Anti-His antibody cross-linked to the MagBeads).

Ordering Information

Cat. No.	Product Name	Size	Price
L00273	Protein A MagBeads	4 ml	$89
L00274	Protein G MagBeads	2 ml	$125
L00277	Protein A/G MagBeads	2 ml	$148