Protein A, G and L Resins

Protein A, protein G and protein L are surface proteins originally found in cell wall of bacteria Staphylococcus aureus, Group G Streptococci and Peptostreptococcus magnus, respectively. Protein A and protein G bind with high affinity and specificity to Fc region of immunoglobulins from many of mammalian species, most notably IgGs. Unlike protein A and G, Protein L binds immunoglobulins through kappa light chain. These proteins have been usually used to purify, immobilize or detect immunoglobulins.

GenScript offers recombinant protein A with non-essential domains removed, recombinant protein G with albumin binding site deleted as well as codon optimized protein L. By pre-coupling these genetically modified proteins to 4% highly cross-linked agarose, GenScrtipt developed excellent antibody affinity chromatography matrix - Protein A, G and L Resins for batch/gravity purification of polyclonal and monoclonal antibody from cell culture supernatants, serum, and ascites at both laboratory and larger scale. With enhanced binding capacity, these resins enable you to get highly purified antibodies with minimal contaminations, resulting in cleaner preparations and greater yields.

Table 1. Properties of the recombinant Protein A, G and L on Resins

	Protein A	Protein G	Protein L
Source	Staphylococcus aureus	Streptococcus	Peptostreptoccus magnus
Molecular weight	43 kDa	22 kDa	42kDa
Numbers of IgG(Ig) binding sites	5	3	5
IgG Binding capacity	>20 mg porcine IgG per ml settled resin	>20 mg sheep IgG per ml settled resin	>15 mg porcine Ig per ml settled resin

Ordering Information

Cat. No.	Product Name	Size	Price
L00210	Protein A Resin	5 ml	$98
L00400	Ultra Protein A Resin	5 ml	$249
L00209	Protein G Resin	5 ml	$129
L00239	Protein L Resin	2.500 ml	$145