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Protein Markers
Protein molecular weight markers
are used to estimate sample molecular weights, to monitor the progress
of an electrophoretic run, and as a positive control for the
electrophoresis and Western conditions. GenScript offers a wide
selection of protein molecular weight markers for protein
electrophoresis and Western blot applications, including unstained,
prestained, peptide, Western-viewable, and other markers. All have been
quality-controlled to assure consistent performance and are available
in convenient package sizes.
Key Features:
- Comprehensive selection: GenScript offers a variety of protein markers for different applications, including SDS-PAGE, Western blot, etc.
- Direct visualization:
GenScript markers allow direct visualization of the MW standard on
Western blots (membrane, X-ray film, chemiluminescent imager).
- Broad molecular weight range: GenScript provides low-molecular weight, mid-range and high molecular weight markers to suit all practical needs.
- His-tagged protein marker: His-tag sequence in the proteins enables detection on western blots using anti-his antibody.
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1.Smart Protein Standard (Mid-Range)
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2.Smart Broad-Range Protein Standard
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The Smart Protein Standard is a mixture of six highly purified recombinant proteins ranging in size from 15 to 100 kD .
Fig1.12% SDS-PAGE gel, Coomassie staining
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Smart Broad-Range Protein Standard, shows nine bands ranging in size from 6 to 220 kD.
Fig2. 4-20% gradient SDS-PAGE gel, Coomassie staining
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3.Smart Prestained Protein Standard
(Mid-Range)
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4.Smart His-Tagged Protein Standard
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The
Smart Prestained Protein Standard (Mid-Range) is a mixture of six
highly purified recombinant proteins ranging in size from 10 to 110
kD,. covalently coupled to blue and red dyes.
Fig3. 4-20% gradient SDS-PAGE gel
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The
Smart His-Tagged Protein Standard is a mixture of six highly purified
recombinant proteins ranging in size from 15 to 115 kD, each containing
a terminal 6xHis tag.
Fig4. Smart His-Tagged Protein Standard detected with Mouse
Anti-His antibody (GenScript, A00186) and Goat Anti-Mouse IgG [HRP]
(GenScript, A00160) (4-25% Tris-glycine gradient gel, PVDF membrane,
chemiluminenscent substrate).
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5.EasyWestern Protein Standard
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Fig5.
EasyWestern Protein Standard is a mixture of five highly purified
recombinant proteins ranging in size from 22 to 120 kD. During Western
blotting, it binds to constant chains of primary and secondary
antibodies, enabling the direct visualization of the standard.The
EasyWestern Protein Standard can bind IgGs from human, mouse, rat,
sheep, goat, and rabbit. However, it is not stained when protein A- or
protein G -based detection methods are used.
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