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Protein Refolding Services

Insoluble aggregates, also called inclusion bodies, are one of the major issues facing protein expression in E. coli. The insoluble aggregates are non-functional but completely block the progress of research into downstream applications. GenScript's protein refolding services provide a solution to this problem.

GenScript offers its Guaranteed Protein Refolding Service for only $1950 for one-liter system. If, for any reason, we are unable to render a given protein soluble, all charges are waived.

GenScript can simultaneously optimize the refolding up to 20 proteins per order. Our confidence is backed by our proprietary refolding technology, which includes high-hydrostatic-pressure techniques, small-molecule additives, and column-refolding methods.

Protein Refolding Services
Protein Refolding Services

Services:

Our typical turnaround time is four weeks. GenScript can provide 30-50% soluble refolded protein at purity of 85% or greater. The final output is dependent on the total amount of target protein comprising the aggregates. The refolded protein is delivered in customized buffer. The major elements of our protein refolding services include the following procedures:

  1. Evaluation of target proteins' biochemical and biophysical properties
  2. Refolding optimizations in the 1L system
  3. The particular refolding strategy is selected based on protein's sequence and the structural properties. Typically, our strategies include the following:
    1. Direct dilution
    2. Dialysis and diafiltration
    3. Small molecular assistants:
      • Denaturant
      • Amino acids
      • Polymers: micellar and liposomal
      • Alcohol
      • Reducing/oxidizing reagents
      • Salts: Na/K/Mg-Cl and (NH4)2SO4
      • Saccharides: glucose, sucrose, and trehalose
    4. Artificial chaperones: detergent and non-detergent
    5. Matrix-assisted methods: SEC (size exclusion chromatography), HIC (hydrophobic interaction chromatography), affinity column and IEC (ion exchange chromatography)
    6. Chaperon-mediated methods: ATP-dependent and ATP-independent
    7. Gradient methods: pH, Tem, hydrostatic pressure, and denaturant

Sample Submission Requirements:

GenScript accepts stab cultures, plasmid DNA, and glycerol and LB cultures as starting materials so long as expression and purification protocols are provided:

MaterialStab CulturePlasmid DNAGlycerol StockLB Culture
Quantity 500 μl 2 μg 1 ml 3 ml

Delivery Specifications:

The typical turnaround is four weeks. GenScript can provide 30-50% soluble refolded protein at the purity of 85% or greater from one-liter expression system, based on total target protein comprising the aggregates. The refolded protein is delivered in customized buffer with the protein purity and identity data determined by Coomassie-stained gels and western blot analyses using Tag or the customer's designated protein-specific antibody (must be provided by the customer).

Quotations and Ordering:

For quotation and questions, you may contact us by email, phone, fax or via our secure web server. Our customer service representatives are available 24 hours, Monday through Friday to assist you.

Please submit the starting materials together with expression and purification protocols to Protein Services, GenScript, 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.

Order by email Order by email: protein@genscript.com
Order by phone Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Order by fax Order by fax: 1-732-210-0262 1-732-885-5878