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Catalog Products » Antibodies » Secondary Antibodies
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Secondary Antibodies

A primary antibody's Fc domain is constant within the same animal species, therefore, a given secondary antibody can be used with any primary antibody of the same isotype and target species. This reduces the cost by labeling only one type of secondary antibody. In addition, secondary antibodies offer increased sensitivity through the signal amplification.

At GenScript, secondary antibodies are purified from the serum of several hosts, including goat, donkey, mouse, rabbit, chicken and sheep. Secondary antibodies are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. The conjugated secondary antibodies can be used for various immunology analysis and protein assays.

GenScript also offers over 1000 highly specific monoclonal and polyclonal primary antibodies. Click here to look for primary antibodies for your protein of interest.

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Cat.No. Host Animal Target Label Display Name Secondary Antibody Specificity
A10001 Goat Mouse IgM (H&L) Unconjugated Mouse IgM (H&L)
A10002 Rabbit Mouse IgM (H&L) Unconjugated Mouse IgM (H&L)
A10003 Goat Mouse IgM (H&L) Fluorescein (FITC) Mouse IgM (H&L)
A10004 Goat Mouse IgM (H&L) Peroxidase (Horseradish) Mouse IgM (H&L)
A10005 Rabbit Mouse IgM (H&L) Unconjugated Mouse IgM (H&L)
A10006 Rabbit Mouse IgM (H&L) Fluorescein (FITC) Mouse IgM (H&L)
A10007 Rabbit Mouse IgM (H&L) Peroxidase (Horseradish) Mouse IgM (H&L)
A10008 Rabbit Bovine IgG F(ab')2 Texas Red® Bovine IgG F(ab')2
A10009 Goat Chicken IgG (H&L) Texas Red® Chicken IgG (H&L)
A10010 Rabbit Chicken IgG (H&L) Texas Red® Chicken IgG (H&L)
A10011 Goat Dog IgG F(c) Texas Red® Dog IgG F(c)
A10012 Rabbit Goat IgG (H&L) Peroxidase (Horseradish) Goat IgG (H&L)
A10013 Rabbit Goat IgG F(c) Peroxidase (Horseradish) Goat IgG F(c)
A10014 Rabbit Goat IgG (H&L) Texas Red® Goat IgG (H&L)
A10015 Donkey Goat IgG (H&L) Peroxidase (Horseradish) Goat IgG (H&L)
A10016 Donkey Goat IgG (H&L) Alkaline Phosphatase (Calf Intestine) Goat IgG (H&L)
A10017 Donkey Goat IgG (H&L) Biotin Goat IgG (H&L)
A10018 Donkey Goat IgG (H&L) Texas Red® Goat IgG (H&L)
A10019 Goat Guinea Pig IgG (H&L) Texas Red® Guinea Pig IgG (H&L)
A10020 Goat Human IgG F(c) Unconjugated Human IgG F(c)
A10021 Goat Human IgG (H&L) Unconjugated Human IgG (H&L)
A10022 Goat Human IgG (H&L) Fluorescein (FITC) Human IgG (H&L)
A10023 Goat Human IgG F(c) Peroxidase (Horseradish) Human IgG F(c)
A10024 Goat Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10025 Goat Human IgG IgA IgM Peroxidase (Horseradish) Human IgG IgA IgM
A10026 Goat Human IgG (H&L) Alkaline Phosphatase (Calf Intestine) Human IgG (H&L)
A10027 Goat Human IgG IgA IgM Alkaline Phosphatase (Calf Intestine) Human IgG IgA IgM
A10028 Goat Human IgG F(c) Biotin Human IgG F(c)
A10029 Goat Human IgG (H&L) Biotin Human IgG (H&L)
A10030 Goat Human IgG (H&L) R-Phycoerythrin (RPE) Human IgG (H&L)
A10031 Goat Human IgG (H&L) Unconjugated Human IgG (H&L)
A10032 Goat Human IgG F(c) Unconjugated Human IgG F(c)
A10033 Goat Human IgG F(ab')2 Unconjugated Human IgG F(ab')2
A10034 Goat Human IgM µ chain Unconjugated Human IgM µ chain
A10035 Goat Human κ chain Unconjugated Human κ chain
A10036 Goat Human λ chain Unconjugated Human λ chain
A10037 Goat Human IgG (H&L) Unconjugated Human IgG (H&L)
A10038 Goat Human IgG (H&L) Fluorescein (FITC) Human IgG (H&L)
A10039 Goat Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10040 Goat Human IgG F(c) Peroxidase (Horseradish) Human IgG F(c)
A10041 Goat Human IgG F(ab')2 Peroxidase (Horseradish) Human IgG F(ab')2
A10042 Goat Human IgM µ chain Peroxidase (Horseradish) Human IgM µ chain
A10043 Goat Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10044 Goat Human IgG F(ab')2 Biotin Human IgG F(ab')2
A10045 Goat Human κ chain Biotin Human κ chain
A10046 Goat Human IgG (H&L) Biotin Human IgG (H&L)
A10047 Goat Human IgG (H&L) Texas Red® Human IgG (H&L)
A10048 Mouse Human IgG (H&L) Unconjugated Human IgG (H&L)
A10049 Rabbit Human IgG (H&L) Unconjugated Human IgG (H&L)
A10050 Rabbit Human IgA Unconjugated Human IgA
A10051 Rabbit Human IgG F(ab')2 Unconjugated Human IgG F(ab')2
A10052 Rabbit Human IgM Fc5µ Unconjugated Human IgM Fc5µ
A10053 Rabbit Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10054 Rabbit Human IgG F(c) Peroxidase (Horseradish) Human IgG F(c)
A10055 Rabbit Human IgG (γ chain) Peroxidase (Horseradish) Human IgG (γ chain)
A10056 Rabbit Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10057 Rabbit Human IgG F(ab')2 Peroxidase (Horseradish) Human IgG F(ab')2
A10058 Rabbit Human IgM Fc5µ Peroxidase (Horseradish) Human IgM Fc5µ
A10059 Rabbit Human IgG (H&L) Alkaline Phosphatase (Calf Intestine) Human IgG (H&L)
A10060 Rabbit Human IgG F(c) Alkaline Phosphatase (Calf Intestine) Human IgG F(c)
A10061 Rabbit Human IgG F(c) Biotin Human IgG F(c)
A10062 Rabbit Human IgA Biotin Human IgA
A10063 Rabbit Human IgG (H&L) Texas Red® Human IgG (H&L)
A10064 Rabbit Human IgG F(c) Texas Red® Human IgG F(c)
A10065 Rabbit Human IgA Texas Red® Human IgA
A10066 Sheep Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10067 Sheep Human IgG (H&L) Biotin Human IgG (H&L)
A10068 Chicken Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10069 Chicken Human IgG (H&L) Texas Red® Human IgG (H&L)
A10070 Goat Mouse IgG + IgM (H&L) Rhodamine (TRITC) Mouse IgG + IgM (H&L)
A10071 Goat Mouse IgG (H&L) Rhodamine (TRITC) Mouse IgG (H&L)
A10072 Goat Mouse IgG (H&L) Rhodamine (TRITC) Mouse IgG (H&L)
A10073 Goat Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10074 Goat Mouse IgG IgA IgM Unconjugated Mouse IgG IgA IgM
A10075 Goat Mouse IgG + IgM (H&L) Fluorescein (FITC) Mouse IgG + IgM (H&L)
A10076 Goat Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A10077 Goat Mouse IgG1 Peroxidase (Horseradish) Mouse IgG1
A10078 Goat Mouse IgG2b Peroxidase (Horseradish) Mouse IgG2b
A10079 Goat Mouse IgG + IgM (H&L) Peroxidase (Horseradish) Mouse IgG + IgM (H&L)
A10080 Goat Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10081 Goat Mouse IgG IgA IgM Peroxidase (Horseradish) Mouse IgG IgA IgM
A10082 Goat Mouse IgG IgA IgM Peroxidase (Horseradish) Mouse IgG IgA IgM
A10083 Goat Mouse IgG (H&L) Alkaline Phosphatase (Calf Intestine) Mouse IgG (H&L)
A10084 Goat Mouse IgG (H&L) Biotin Mouse IgG (H&L)
A10085 Goat Mouse IgG (H&L) R-Phycoerythrin (RPE) Mouse IgG (H&L)
A10086 Goat Mouse IgG (H&L) Texas Red® Mouse IgG (H&L)
A10087 Goat Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10088 Goat Mouse IgG F(c) Unconjugated Mouse IgG F(c)
A10089 Goat Mouse IgM µ chain Unconjugated Mouse IgM µ chain
A10090 Goat Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10091 Goat Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A10092 Goat Mouse IgG F(ab')2 Fluorescein (FITC) Mouse IgG F(ab')2
A10093 Goat Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10094 Goat Mouse IgG F(c) Peroxidase (Horseradish) Mouse IgG F(c)
A10095 Goat Mouse IgG F(ab')2 Peroxidase (Horseradish) Mouse IgG F(ab')2
A10096 Goat Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10097 Goat Mouse IgG (H&L) Alkaline Phosphatase (Calf Intestine) Mouse IgG (H&L)
A10098 Goat Mouse IgG (H&L) Alkaline Phosphatase (Calf Intestine) Mouse IgG (H&L)
A10099 Goat Mouse IgG (H&L) Biotin Mouse IgG (H&L)
A10100 Goat Mouse IgG (H&L) Texas Red® Mouse IgG (H&L)
A10101 Rabbit Mouse IgG (H&L) Rhodamine (TRITC) Mouse IgG (H&L)
A10102 Rabbit Mouse κ chain Rhodamine (TRITC) Mouse κ chain
A10103 Rabbit Mouse IgG (γ 1, 2a, 2b and 3 chain) Peroxidase (Horseradish) Mouse IgG (γ 1, 2a, 2b and 3 chain)
A10104 Rabbit Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10105 Rabbit Mouse IgG F(c) Unconjugated Mouse IgG F(c)
A10106 Rabbit Mouse IgM µ chain Unconjugated Mouse IgM µ chain
A10107 Rabbit Mouse κ chain Unconjugated Mouse κ chain
A10108 Rabbit Mouse λ chain Unconjugated Mouse λ chain
A10109 Rabbit Mouse IgG (γ chain) Unconjugated Mouse IgG (γ chain)
A10110 Rabbit Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10111 Rabbit Mouse IgG1 Unconjugated Mouse IgG1
A10112 Rabbit Mouse IgG2a Unconjugated Mouse IgG2a
A10113 Rabbit Mouse IgG2b Unconjugated Mouse IgG2b
A10114 Rabbit Mouse IgG3 Unconjugated Mouse IgG3
A10115 Rabbit Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A10116 Rabbit Mouse IgG1 Fluorescein (FITC) Mouse IgG1
A10117 Rabbit Mouse IgG2a Fluorescein (FITC) Mouse IgG2a
A10118 Rabbit Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10119 Rabbit Mouse IgG F(c) Peroxidase (Horseradish) Mouse IgG F(c)
A10120 Rabbit Mouse κ chain Peroxidase (Horseradish) Mouse κ chain
A10121 Rabbit Mouse IgG (γ chain) Peroxidase (Horseradish) Mouse IgG (γ chain)
A10122 Rabbit Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10123 Rabbit Mouse IgG1 Peroxidase (Horseradish) Mouse IgG1
A10124 Rabbit Mouse IgG2a Peroxidase (Horseradish) Mouse IgG2a
A10125 Rabbit Mouse IgG2b Peroxidase (Horseradish) Mouse IgG2b
A10126 Rabbit Mouse IgG3 Peroxidase (Horseradish) Mouse IgG3
A10127 Rabbit Mouse IgG (H&L) Alkaline Phosphatase (Calf Intestine) Mouse IgG (H&L)
A10128 Rabbit Mouse IgA Alkaline Phosphatase (Calf Intestine) Mouse IgA
A10129 Rabbit Mouse κ chain Alkaline Phosphatase (Calf Intestine) Mouse κ chain
A10130 Rabbit Mouse λ chain Alkaline Phosphatase (Calf Intestine) Mouse λ chain
A10131 Rabbit Mouse IgG (H&L) Alkaline Phosphatase (Calf Intestine) Mouse IgG (H&L)
A10132 Rabbit Mouse IgG1 Alkaline Phosphatase (Calf Intestine) Mouse IgG1
A10133 Rabbit Mouse IgG2a Alkaline Phosphatase (Calf Intestine) Mouse IgG2a
A10134 Rabbit Mouse IgG (H&L) Biotin Mouse IgG (H&L)
A10135 Rabbit Mouse IgG F(c) Biotin Mouse IgG F(c)
A10136 Rabbit Mouse IgM µ chain Biotin Mouse IgM µ chain
A10137 Rabbit Mouse IgG1 Biotin Mouse IgG1
A10138 Rabbit Mouse IgG2a Biotin Mouse IgG2a
A10139 Rabbit Mouse IgG F(c) Texas Red® Mouse IgG F(c)
A10140 Rabbit Mouse IgA Texas Red® Mouse IgA
A10141 Rabbit Mouse IgG (γ chain) Texas Red® Mouse IgG (γ chain)
A10142 Sheep Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10143 Sheep Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A10144 Sheep Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10145 Sheep Mouse IgG (H&L) Texas Red® Mouse IgG (H&L)
A10146 Donkey Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10147 Donkey Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A10148 Donkey Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10149 Donkey Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10150 Donkey Mouse IgG (H&L) Biotin Mouse IgG (H&L)
A10151 Donkey Mouse IgG (H&L) Texas Red® Mouse IgG (H&L)
A10152 Donkey Mouse IgG (H&L) Texas Red® Mouse IgG (H&L)
A10153 Chicken Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10154 Chicken Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10155 Goat Rabbit IgG (H&L) Rhodamine (TRITC) Rabbit IgG (H&L)
A10156 Goat Rabbit IgG (H&L) Rhodamine (TRITC) Rabbit IgG (H&L)
A10157 Goat Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10158 Goat Rabbit IgG (H&L) Fluorescein (FITC) Rabbit IgG (H&L)
A10159 Goat Rabbit IgG F(c) Rhodamine (TRITC) Rabbit IgG F(c)
A10160 Goat Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10161 Goat Rabbit IgG (H&L) Alkaline Phosphatase (Calf Intestine) Rabbit IgG (H&L)
A10162 Goat Rabbit IgG (H&L) Biotin Rabbit IgG (H&L)
A10163 Goat Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10164 Goat Rabbit IgG F(c) Unconjugated Rabbit IgG F(c)
A10165 Goat Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10166 Goat Rabbit IgG F(c) Unconjugated Rabbit IgG F(c)
A10167 Goat Rabbit IgG (H&L) Fluorescein (FITC) Rabbit IgG (H&L)
A10168 Goat Rabbit IgG (H&L) Fluorescein (FITC) Rabbit IgG (H&L)
A10169 Goat Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10170 Goat Rabbit IgG F(c) Peroxidase (Horseradish) Rabbit IgG F(c)
A10171 Goat Rabbit IgG F(ab')2 Peroxidase (Horseradish) Rabbit IgG F(ab')2
A10172 Goat Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10173 Goat Rabbit IgG F(c) Peroxidase (Horseradish) Rabbit IgG F(c)
A10174 Goat Rabbit IgG (H&L) Alkaline Phosphatase (Calf Intestine) Rabbit IgG (H&L)
A10175 Goat Rabbit IgG F(c) Alkaline Phosphatase (Calf Intestine) Rabbit IgG F(c)
A10176 Goat Rabbit IgG (H&L) Alkaline Phosphatase (Calf Intestine) Rabbit IgG (H&L)
A10177 Goat Rabbit IgG F(c) Alkaline Phosphatase (Calf Intestine) Rabbit IgG F(c)
A10178 Goat Rabbit IgG (H&L) Biotin Rabbit IgG (H&L)
A10179 Goat Rabbit IgG F(c) Biotin Rabbit IgG F(c)
A10180 Goat Rabbit IgG F(c) Biotin Rabbit IgG F(c)
A10181 Goat Rabbit IgG (H&L) Texas Red® Rabbit IgG (H&L)
A10182 Goat Rabbit IgG F(c) Texas Red® Rabbit IgG F(c)
A10183 Goat Rabbit IgG F(c) Texas Red® Rabbit IgG F(c)
A10184 Mouse Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10185 Sheep Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10186 Donkey Rabbit IgG (H&L) Rhodamine (TRITC) Rabbit IgG (H&L)
A10187 Donkey Rabbit IgG F(c) Unconjugated Rabbit IgG F(c)
A10188 Donkey Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10189 Donkey Rabbit IgG F(c) Fluorescein (FITC) Rabbit IgG F(c)
A10190 Donkey Rabbit IgG (H&L) Fluorescein (FITC) Rabbit IgG (H&L)
A10191 Donkey Rabbit IgG F(c) Peroxidase (Horseradish) Rabbit IgG F(c)
A10192 Donkey Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10193 Donkey Rabbit IgG (H&L) Alkaline Phosphatase (Calf Intestine) Rabbit IgG (H&L)
A10194 Donkey Rabbit IgG F(c) Biotin Rabbit IgG F(c)
A10195 Donkey Rabbit IgG (H&L) Biotin Rabbit IgG (H&L)
A10196 Donkey Rabbit IgG (H&L) Texas Red® Rabbit IgG (H&L)
A10197 Donkey Rabbit IgG (H&L) Fluorescein (FITC) Rabbit IgG (H&L)
A10198 Donkey Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10199 Donkey Rabbit IgG (H&L) Alkaline Phosphatase (Calf Intestine) Rabbit IgG (H&L)
A10200 Donkey Rabbit IgG (H&L) Biotin Rabbit IgG (H&L)
A10201 Donkey Rabbit IgG (H&L) Texas Red® Rabbit IgG (H&L)
A10202 Chicken Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10203 Goat Rat IgG (H&L) Unconjugated Rat IgG (H&L)
A10204 Goat Rat IgG (H&L) Peroxidase (Horseradish) Rat IgG (H&L)
A10205 Goat Rat IgG (H&L) Biotin Rat IgG (H&L)
A10206 Goat Rat IgG (H&L) R-Phycoerythrin (RPE) Rat IgG (H&L)
A10207 Goat Rat IgG F(c) Unconjugated Rat IgG F(c)
A10208 Goat Rat IgM µ chain Unconjugated Rat IgM µ chain
A10209 Goat Rat IgG (H&L) Fluorescein (FITC) Rat IgG (H&L)
A10210 Goat Rat IgG F(c) Fluorescein (FITC) Rat IgG F(c)
A10211 Goat Rat IgG (H&L) Peroxidase (Horseradish) Rat IgG (H&L)
A10212 Goat Rat IgG F(c) Peroxidase (Horseradish) Rat IgG F(c)
A10213 Goat Rat IgA Peroxidase (Horseradish) Rat IgA
A10214 Goat Rat IgM µ chain Peroxidase (Horseradish) Rat IgM µ chain
A10215 Goat Rat IgG (H&L) Alkaline Phosphatase (Calf Intestine) Rat IgG (H&L)
A10216 Goat Rat IgG (H&L) Biotin Rat IgG (H&L)
A10217 Goat Rat IgG F(c) Biotin Rat IgG F(c)
A10218 Rabbit Rat IgG (H&L) Unconjugated Rat IgG (H&L)
A10219 Rabbit Rat IgG (H&L) Fluorescein (FITC) Rat IgG (H&L)
A10220 Rabbit Rat IgG (H&L) Peroxidase (Horseradish) Rat IgG (H&L)
A10221 Rabbit Rat IgG (H&L) Biotin Rat IgG (H&L)
A10222 Rabbit Rat IgG (H&L) Texas Red® Rat IgG (H&L)
A10223 Sheep Rat IgG (H&L) Unconjugated Rat IgG (H&L)
A10224 Donkey Rat IgG (H&L) Unconjugated Rat IgG (H&L)
A10225 Donkey Rat IgG (H&L) Peroxidase (Horseradish) Rat IgG (H&L)
A10226 Donkey Rat IgG (H&L) Texas Red® Rat IgG (H&L)
A10227 Rabbit Sheep IgG (H&L) Texas Red® Sheep IgG (H&L)
A10228 Goat Swine IgG (H&L) Texas Red® Swine IgG (H&L)
A10229 Goat Monkey IgA Unconjugated Monkey IgA
A10230 Goat Monkey IgG (γ chain) Unconjugated Monkey IgG (γ chain)
A10231 Goat Monkey IgG IgA IgM Unconjugated Monkey IgG IgA IgM
A10232 Goat Monkey IgA Fluorescein (FITC) Monkey IgA
A10233 Goat Monkey IgG (γ chain) Fluorescein (FITC) Monkey IgG (γ chain)
A10234 Goat Monkey IgA Peroxidase (Horseradish) Monkey IgA
A10235 Goat Monkey IgM µ chain Peroxidase (Horseradish) Monkey IgM µ chain
A10236 Goat Monkey IgG (γ chain) Peroxidase (Horseradish) Monkey IgG (γ chain)
A10237 Goat Monkey IgG IgA IgM Peroxidase (Horseradish) Monkey IgG IgA IgM
A10238 Goat Monkey IgA Biotin Monkey IgA
A10239 Goat Monkey IgG (γ chain) Biotin Monkey IgG (γ chain)
A10240 Goat Armenian Hamster IgG (H&L) Peroxidase (Horseradish) Armenian Hamster IgG (H&L)
A10241 Donkey Goat IgG (H&L) Peroxidase (Horseradish) Goat IgG (H&L)
A10242 Donkey Goat IgG (H&L) R-Phycoerythrin (RPE) Goat IgG (H&L)
A10243 Goat Human IgG IgA IgM Unconjugated Human IgG IgA IgM
A10244 Goat Human IgG IgA IgM Peroxidase (Horseradish) Human IgG IgA IgM
A10245 Goat Human IgG IgA IgM Alkaline Phosphatase (Calf Intestine) Human IgG IgA IgM
A10246 Goat Human IgA Unconjugated Human IgA
A10247 Goat Human κ chain Unconjugated Human κ chain
A10248 Goat Human IgG (H&L) Unconjugated Human IgG (H&L)
A10249 Goat Human IgG F(c) Unconjugated Human IgG F(c)
A10250 Goat Human κ chain Fluorescein (FITC) Human κ chain
A10251 Goat Human λ chain Fluorescein (FITC) Human λ chain
A10252 Goat Human IgA Peroxidase (Horseradish) Human IgA
A10253 Goat Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10254 Goat Human IgG F(c) Peroxidase (Horseradish) Human IgG F(c)
A10255 Goat Human IgG F(c) Alkaline Phosphatase (Calf Intestine) Human IgG F(c)
A10256 Goat Human IgG F(c) Biotin Human IgG F(c)
A10257 Goat Human IgG (H&L) R-Phycoerythrin (RPE) Human IgG (H&L)
A10258 Goat Human IgG F(c) R-Phycoerythrin (RPE) Human IgG F(c)
A10259 Goat Human IgG F(c) Texas Red® Human IgG F(c)
A10260 Donkey Human IgG (H&L) Texas Red® Human IgG (H&L)
A10261 Goat Mouse IgG F(c) Rhodamine (TRITC) Mouse IgG F(c)
A10262 Goat Mouse IgG F(c) Unconjugated Mouse IgG F(c)
A10263 Goat Mouse IgG F(ab')2 Peroxidase (Horseradish) Mouse IgG F(ab')2
A10264 Goat Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10265 Goat Mouse IgG (H&L) Biotin Mouse IgG (H&L)
A10266 Goat Mouse IgG F(c) R-Phycoerythrin (RPE) Mouse IgG F(c)
A10267 Rabbit Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10268 Rabbit Mouse IgG (H&L) R-Phycoerythrin (RPE) Mouse IgG (H&L)
A10269 Donkey Mouse IgG (H&L) Texas Red® Mouse IgG (H&L)
A10270 Goat Rabbit IgG (H&L) Texas Red® Rabbit IgG (H&L)
A10271 Goat Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10272 Goat Rabbit IgG F(ab')2 Unconjugated Rabbit IgG F(ab')2
A10273 Goat Rabbit IgG F(c) Peroxidase (Horseradish) Rabbit IgG F(c)
A10274 Goat Rabbit IgG (H&L) Texas Red® Rabbit IgG (H&L)
A10275 Goat Rabbit IgG F(ab')2 Texas Red® Rabbit IgG F(ab')2
A10276 Donkey Rabbit IgG (H&L) R-Phycoerythrin (RPE) Rabbit IgG (H&L)
A10277 Goat Rat IgG F(c) Peroxidase (Horseradish) Rat IgG F(c)
A10278 Goat Rat IgG (H&L) Peroxidase (Horseradish) Rat IgG (H&L)
A10279 Donkey Goat IgG (H&L) Peroxidase (Horseradish) Goat IgG (H&L)
A10280 Goat Human IgG (H&L) Unconjugated Human IgG (H&L)
A10281 Goat Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A10282 Rabbit Human IgG (H&L) Unconjugated Human IgG (H&L)
A10283 Goat Mouse IgG (H&L) Unconjugated Mouse IgG (H&L)
A10284 Goat Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A10285 Goat Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A10286 Goat Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A10287 Goat Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A10288 Goat Rabbit IgG (H&L) Texas Red® Rabbit IgG (H&L)
A01860-200 Rabbit Camelid VHH Unconjugated Camelid VHH
A01861-200 Rabbit Camelid VHH Peroxidase (Horseradish) Camelid VHH
A00098 Goat Rabbit IgG (H&L) Peroxidase (Horseradish) Rabbit IgG (H&L)
A00131 Goat Rabbit IgG (H&L) Unconjugated Rabbit IgG (H&L)
A00160 Goat Mouse IgG (H&L) Peroxidase (Horseradish) Mouse IgG (H&L)
A01318 Goat Mouse IgG (H&L) Fluorescein (FITC) Mouse IgG (H&L)
A00165 Goat Chicken IgY (H&L) Peroxidase (Horseradish) Chicken IgY (H&L)
A00166 Goat Human IgG (H&L) Peroxidase (Horseradish) Human IgG (H&L)
A00167 Goat Rat IgG (H&L) Peroxidase (Horseradish) Rat IgG (H&L)
A00178 Donkey Goat IgG Peroxidase (Horseradish) Goat IgG
A01827-200 Mouse Rabbit IgG Peroxidase (Horseradish) Rabbit IgG
A01856-200 Mouse Rabbit IgG F(c) Peroxidase (Horseradish) Rabbit IgG F(c)
A01855-200 Mouse Human IgG F(ab) Peroxidase (Horseradish) Human IgG F(ab)
A01854-200 Mouse Human IgG F(c) Peroxidase (Horseradish) Human IgG F(c)

Step 1. The target species of the secondary antibody need to match the host of the primary antibody

Step 2. The immunogen of the secondary antibody need to match the class (isotype), subclass or the fragment of the primary antibody. Select an appropriate host species for the secondary antibody.

Example:

Immunoglobulin (Ig) Types of Mammals

Ig Class (Isotype) Subclass Heavy Chain Light Chain Complexes
Ig A Ig A1, Ig A2 α1, α2 κ or λ dimer
Ig D   δ monomer
Ig E   ε monomer
Ig G Ig G1, Ig G2a, Ig G2b, Ig G3, Ig G4 γ1, γ2, γ3, γ4 monomer
Ig M   μ pentamer

Step 3. Choose the label of the secondary antibody according to your application.

Application Label Type Label Options
ELISA Enzyme Biotin,
Alkaline Phosphatase (AP),
Horseradish Peroxidase (HRP)
Western Blot (WB)
Flow Cytometry Fluorochrome R-Phycoerythrin(RPE),
Fluorescein (FITC),
Texas Red®,
Rhodamine (TRITC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
FLISA
Immunohistochemistry (IHC) Enzyme/Fluorochrome Biotinylated secondary antibodies – avidin/streptavidin conjugated to enzyme/fluorochrome

Step 4. Consider other specificity issues of the secondary antibody

  • Antibody fragment

    If working with tissues or cells that have Fc receptors (spleen, blood, hematopoietic cells, leukocytes, etc.), choose a F(ab) or F(ab')2 fragment to eliminate non-specific binding. 
    F(ab')2 fragment secondary antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact the hinge region. Two F(ab) fragments and an Fc fragment are generated if digested by papain.

  • Pre-adsorbed (also cross-adsorbed)

    The pre-adsorption process reduces the risk of cross reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. The process involves passing the secondary antibody over immobilized serum proteins from potentiAlly cross reactive species.
    Example: Goat Anti-Mouse IgG (H&L) [TRITC], pAb (Cat.no A10071) is pre-adsorbed and has Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins.

    • Species Abbreviation
      Bovine Bv
      Chicken Ck
      Donkey Do
      Goat Gt
      Guinea Pig GP
      Hamster Hm
      Human Hu
    • Species Abbreviation
      Monkey Nhp
      Horse Eq
      Mouse Ms
      Rabbit Rb
      Rat Rt
      Sheep Ov
      Pig Po
  • Clonality

    Polyclonal antibodies are sufficient for most needs. Monoclonal antibodies can minimize background, cross reactivity and improve the lot-to-lot consistency.

    Example:

    Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (Cat.no A01827-200) only recognizes native rabbit primary IgG antibodies and does not react with denatured or reduced rabbit IgG heavy or light chain. Immunoprecipitation and Western blots often suffer from primary antibody heavy and light chain contamination at approximately 50 and 25 kDa, which is caused by poor specificity of conventional polyclonal secondary antibodies derived from goat or other species.

  1. Is Catalog No. A00166 (Goat Anti-Human IgG Antibody (H&L) [HRP], pAb) specific for human IgG? Does it cross-react with non-human primate or rodents?

    Catalog No. A00166 (Goat Anti-Human IgG Antibody (H&L) [HRP], pAb) recognizes human IgG, but it has not yet been tested for cross-reactivity with other species.

  2. Does Catalog No. A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) cross react with rabbit?

    Catalog. No A00160 (Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb) has not yet been tested for cross-reactivity with rabbit.


   
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