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Secondary Antibody Coated MagBeads

Secondary Antibody Coated MagBeads Workflow

GenScript Secondary Antibody Coated MagBeads (e.g. Goat Anti-Rabbit IgG MagBeads and Donkey Anti-Goat IgG MagBeads) are pre-coupled with purified secondary antibodies and displays strong specificity and high affinity to corresponding IgGs, allowing for maximum flexibility of your protein and antibody purification. These MagBeads can be easily used for immunoprecipitation and quick, small–scale purification of IgGs. Additionally a specific primary antibody can also be added to the MagBeads to isolate your target protein using a direct or indirect method. The direct method is preferred when the target protein is abundant: the primary antibody is firstly bound to the GenScript MagBeads, the Ab-bound beads are then incubated with your sample to capture the target protein. The indirect method is well suited when the primary antibody has a poor affinity for the target or when the target is in low-abundance: the primary antibody is firstly incubated with your sample to form an Ab-antigen complex; this complex is then captured by adding GenScript MagBeads to your sample, followed by magnetic separation.

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