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Semi-custom Monoclonal Antibody Production Services

Semi-custom Monoclonal Antibody Production Services

Semi-custom Monoclonal Antibody Production Services

GenScript’s semi-custom monoclonal antibody production services allow quick and easy construction of your desired monoclonal antibody production protocol from a series of pre-defined units. Your choices include protein or peptide antigen, express or traditional immunization protocol, ImmunoPlus™ technology, additional screening and testing, as well as the scale of antibody production. With pre-defined units, you can quickly build your monoclonal antibody production protocol by choosing the desired units based on your research need. Designing our custom monoclonal antibody production protocol can be as easy as stacking blocks. Let our antibody production experts do the rest.

Key Features of Semi-custom Monoclonal Antibody Production Services

Semi-custom Monoclonal Antibody Production Services Diagram

Semi-custom Monoclonal Antibody Production Services Illustration

Semi-custom Monoclonal Antibody Production Services Details

  Cat# Name Service
Description
Final Deliverables Time (days)
Step 1:
Antigen preparation
SC1744AG
Peptide antigen preparation for immunization
Peptide synthesis and
KLH/BSA conjugation
2 mg free peptide
21
SC1745AG
Protein antigen preparation for immunization
Protein antigen expression and purification in bacterial expression system
200 ug protein
42
SC1748
ImmunoPlus Modification
Our proprietary technology to break immune tolerance
n/a
3
Step 2:
Immunization & Cell Fusion
SC1744ME
Express Immunization + Cell fusion (for peptide antigen)
  • Immunize 5 Balb/c mice or 3 Wistar rats with MonoExpress™ immunization strategy;
  • 1-2 rounds of cell fusions;
  • Screening with 10-15 96-well plate (from 2x10E4 clones) by indirect ELISA.
Deliver supernatants of up to 10 clones
30
SC1744TD
Traditional Immunization + Cell fusion (for peptide antigen)
  • Immunize 5 Balb/c mice or 3 Wistar rats with traditional immunization strategy;
  • 1-2 rounds of cell fusions;
  • Screening with 10-15 96-well plate (from 2x10E4 clones) by indirect ELISA.
Deliver supernatants of up to 10 clones
74
SC1745ME
Express Immunization + Cell fusion (for protein antigen)
  • Immunize 5 Balb/c mice or 3 Wistar rats with MonoExpress™ immunization strategy;
  • 1-2 rounds of cell fusions;
  • Screening with 10-15 96-well plate (from 2x10E4 clones) by indirect ELISA.
Deliver supernatants of up to 20 clones
30
SC1745TD
Traditional Immunization + Cell fusion (for protein antigen)
  • Immunize 5 Balb/c mice or 3 Wistar rats with traditional immunization strategy;
  • 1-2 rounds of cell fusions;
  • Screening with 10-15 96-well plate (from 2x10E4 clones) by indirect ELISA.
Deliver supernatants of up to 20 clones
74
Step 3:
Subcloning & Ab purification
SC1744AB/SC1744C
Subcloning & Ab purification (for peptide antigen)
  • 2 positive primary clones selected by customer will be subcloned by limiting dilution
  • 1 subclone will be expanded for each primary clone.
  • 2 positive clones selected by customer;
  • 0.2 mg purified antibody per clone;
  • 2 vials of frozen cell per clone
56
SC1745AB/SC1745C
Subcloning & Ab purification (for protein antigen)
  • 2 positive primary clones selected by customer will be subcloned by limiting dilution
  • 1 subclone will be expanded for each primary clone.
  • 2 positive clones selected by customer;
  • 0.2 mg purified antibody per clone;
  • 2 vials of frozen cell per clone
56

Cat# Service Description Note
Additional Services
SC1221
Additional clones + 0.2 mg purified Ab per clone
 
SC1662
 
SC1716
2 mg purified antibody/clone
 
SC1223
Additional antigen screening and testing
Only one SC1223 could be added
SC1110
Ascites Production in Balb/c mice
 
SC1117
Roller bottle cell culture and purification
 
SC1034
Extended Protocol
 
SC1483
1-5 mg purified antibody/clone
 


Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.

Semi-custom Monoclonal Antibody Production Services

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Semi-custom Monoclonal Antibody Production Services

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