Large-Scale DNA Sequencing
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DNA sequencing is an important part of genetic analysis, with significant consequences not only for basic research into fundamental biological processes such as gene expression, gene regulation, and phylogenetics, but also for applied research such as diagnostics, forensics, and gene therapy.
GenScript offers quick, high-quality, cost-effective DNA sequencing services. Our sequencing service includes genomic DNA sequencing, PCR fragment sequencing, and plasmid DNA sequencing. All processes employ state-of-the-art equipment, including the ABI 3730xl DNA Analyzer. Our service is compatible with studies involving mutagenesis, polymorphisms, mutant screening, construct generation, genome sequencing, and the cloning of new genes.
We offer a free trial of one sequencing run, with typical readout of about 800 bases.
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Key Features:
- Guaranteed accuracy and coverage
- Access to standard primers
- Full bioinformatics technical support
- Comprehensive packages
- Competitive prices
- Fast turnaround time
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Service Specifications:
Our sequencing services include primer walking, FDA-quality DNA sequencing, and large-scale DNA sequencing. The general sequencing service package includes PCR amplification, amplicon cleanup, sequencing, and data processing. The final delivery package includes sequencing data and full QC reports. Our service offerings are flexible to meet any of our customers' needs.
Primer Walking
Primer walk sequencing is the method of choice for sequencing DNA fragments of between 1.3 and 7 kb, and it is applicable to PCR amplicons, plasmid inserts, or even entire plasmids. GenScript's primer walk sequencing service is divided into single-strand primer walks and double-strand primer walks according to the customers' requirements. Our service package includes the following:
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Primer design and synthesis
Sequencing
Base calling and quality scoring
Sequencing data delivery/retrieval via secure web server |
Sequencing reactions
Dye terminator removal
DNA sequence finishing and assembly |
FDA-Quality DNA Sequencing
GenScript performs all FDA submission services in a fully compliant laboratory environment that exceeds the FDA's standards for quality, reliability, and consistency. Our service guarantees the following:
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Detailed sequencing strategy
Minimum fourfold repeats |
Complete double-stranded coverage
Absolutely confidentiality |
Our final delivery package includes the original electropherograms, consensus sequence, and QC reports. Upon request, we can provide sequence discrepancy reports comparing observed vs expected sequences and any effects of insertions or deletions on amino acid sequence.
Large-Scale Sequencing
GenScript has many years of experience in large-scale sequencing. Our large-scale sequencing service is de novo, which does not require any pre-existing information about the sequence. Our large-scale DNA sequencing service package includes the following:
- High-throughput single- and double-stranded sequencing
- High-throughput DNA extraction/purification (96- or 384-well format)
- Data delivery via our secure web server
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- Library sequencing (BAC, PAC, Cosmid, EST)
- DNA sequence finishing and assembly
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The final delivery package includes the original electropherograms, consensus sequence, and QC reports.
Sample Submission Requirements:
Our requirements for sample submission are listed in the table below:
| Template |
Concentration |
Volume/Reaction |
Solvent |
| Genomic DNA |
50-100 ng/ul |
≥5 ul
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ddH2O or 10 mM Tris (without EDTA)
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| PCR Fragments |
20 ng DNA/100 bp |
≥8 ul
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| Plasmid DNA |
200 - 500 ng/reaction |
≥8 ul
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Please Note:
- Our large-scale sequencing service currently accepts orders of 192 samples or more.
- For combined submissions of templates and primers, the templates must meet our sample submission requirements, and the primers must be submitted in quantities of at least 20 ul (10 pmol/ul or 10 ng/ul) each.
- Standard primers [M13(-21), M13 rev, SP6, T7, T3, BGH, GL2, RV3, NCMV30, CCMV24] are available at no extra charge.
Additional Services:
- GenScript synthesizes primers and oligos at a rate as low as $0.20 per base and designs primers for PCR and sequencing at no additional cost.
- GenScript offers highly cost-effective DNA extraction and purification services.
Pricing:
| Service No. |
Service |
Price |
| SC1201 |
Genomic DNA Sequencing |
$7.00/sample |
| SC1202 |
PCR Fragments and Plasmid DNA Sequencing |
$5.00/sample |
| SC1203 |
Plasmid DNA Extraction from E. coli
(E. coli Recovery, Plasmid DNA Extraction) |
Extra $3.00/sample |
Ordering and Quotations:
 Orders can be placed by phone, email, or fax with a formal PO (Purchase Order) or credit card.
To submit samples, please download and complete Sequencing Order Form and send it to gene@genscript.com. Please also attach a completed hard copy to your samples and send them to the address specified on the form.
For questions and quotation requests, please use our secure web server. However, you may also contact us by email at gene@genscript.com, phone, or fax.
Our customer service representatives are available 24 hours, Monday through Friday to assist you.
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Order by email: gene@genscript.com |
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Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Order by fax: 1-732-210-0262 1-732-885-5878 |
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